人巨细胞病毒感染对人脑血管平滑肌细胞AT受体基因表达的影响  

The Expression of AT Receptors in Smooth Muscle Cells of Human Brain Artery after HCMV Infection

作　　者：王佳伟[1] 脱厚珍[1] 王瑞金[1] 李伟荣[1] 冯子敬[1] 陈珺[1] 王得新[1] 

机构地区：[1]首都医科大学附属北京友谊医院神经内科,100050

出　　处：《中国临床神经科学》2007年第1期8-11,共4页Chinese Journal of Clinical Neurosciences

基　　金：北京市科技新星计划资助(2005B42)

摘　　要：目的:探讨血管紧张素受体1、2(AT1,AT2)及人巨细胞病毒(HCMV)感染在动脉粥样硬化过程中的作用。方法:建立HCMV感染细胞模型,采用RT-PCR等技术研究在更昔洛韦(GCV)和AT1、AT2受体拮抗剂作用下,HCMV对体外培养人脑动脉血管平滑肌细胞AT1和AT2受体基因表达的影响。结果:HCMV感染体外培养的人脑血管平滑肌细胞上调了AT1和AT2受体的基因表达,AT1和AT2受体拮抗剂及GCV能够抑制HCMV感染对人脑血管平滑肌AT1和AT2受体的激活作用。结论:HCMV感染引起人脑血管平滑肌细胞增生可能由于AT1和AT2受体基因表达的激活。Aim： To study the pathogenesis of atherosclerosis by the expression of angiotensin （AT） receptors in vascular smooth muscle cells （VSMCs） of human brain artery after HCMV infection. Methods： By using HCMV AD169 strain infected VSMCs in vitro, to establish the cell model of viral infection. The expression of AT receptors in VSMCs was undergoing HCMV infection, with RT-PCR technique. Results： In comparison with control group （no infection）, HCMV infection could enhance the gene expression of AT1 and AT2 receptors of VSMCs in vitro, at 5 days after infection the gene expression of the AT1 and AT2 receptors was much higher than that of 3 days and 1 days, respectively. GCV group showed stronger antiviral activity in the HCMV infected VSMCs. Conclusion： HCMV infection might excite the gene expression of AT1 and AT2 receptor simultaneously. This project provides some new experimental evidence for the pathogenesis of atherosclerosis.

关 键 词：人巨细胞病毒 平滑肌细胞 血管紧张素受体 

分 类 号：R743[医药卫生—神经病学与精神病学] Q189[医药卫生—临床医学]