Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize  

作　　者：WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu 

机构地区：[1]National Maize Improvement Center of China, China Agriculture University, Beijing 100094, China [2]Maize Research Center, Beijing Academy of Agricultural & Forestry Sciences, Beijing 100097, China

出　　处：《Chinese Science Bulletin》2007年第2期215-223,共9页

基　　金：the Beijing Agricultural Innovative Platform-Beijing Natural Science Fund Program (Grant No. YZPT02-06)

摘　　要：In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome.In the current study, 1900 maize simple sequence repeat （SSR） primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer

关 键 词：基因序列 玉米 分子标记 遗传型 

分 类 号：S513[农业科学—作物学]