Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment  

作　　者：Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan 

机构地区：[1]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China [2]College of Landscape and art, Jiangxi Agricultural University, Nanchang 330045, China [3]Institute of Genetics and developmental biology, Chinese Academy of Sciences, Beijing 100101, China [4]Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China [5]Microbiology Institute, Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Chinese Science Bulletin》2007年第2期224-230,共7页

摘　　要：A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.A JERF36 regulation gene, a selection marker gene （NPT-Ⅱ）, and the foreign genes levansucrase （SacB）, Vitreoscilla hemoglobin （vgb）, and Binary coleopterus insect resistance （BtCry3A＋OC-I） were co-transferred into Populus xeuramericana ＇Guariento＇ using biolistic bombardment; 25 kanamycin resistant plants were obtained, The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P, xeuramericana ＇Guariento＇ and 5 genes were all transferred into 7 poplar plants, The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land,

关 键 词：森林 生态系统 全球变暖 二氧化碳 植被 

分 类 号：X16[环境科学与工程—环境科学]