卵胞质移植、克隆与逆向克隆  

Ooplasmic transfer, cloning and reverse cloning

作　　者：仇欣霞[1] 陈英华[1] 赵冰雷[1] 董为人[1] 

机构地区：[1]南方医科大学组织学与胚胎学教研室,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2007年第7期1361-1364,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:最近人类卵细胞间的胞质移植被作为人类辅助生殖生物技术的手段并成为研究热点。本文回顾了该领域的研究进展和存在的问题,并以此为基础提出“逆向克隆技术”这一新概念。资料来源:应用计算机检索PUBMED 1998-01/2006-12期间的相关文章,检索词为“ooplasmic transfer,mitochondria heteroplasmy,animal cloning”,并限定文章语言种类为English。同时计算机检索万方数据库1998-01/2006-12期间的相关文章,检索词为“胞质转移,线粒体异质性,动物克隆”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与卵胞质移植、克隆或逆向克隆研究相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到126篇相关文献,31篇文献符合纳入标准,排除的95篇文献为内容陈旧或重复。符合纳入标准的31篇文献中,17篇涉及卵胞质移植,13篇涉及动物克隆,1篇涉及逆克隆。资料综合:人类的生殖技术领域发展迅速,卵胞质移植技术得到广泛应用。研究表明,卵胞质对受精和胚胎发育具有重要作用,其中线粒体与受精和胚胎发育关系最为密切。大量实验对细胞质与细胞核的相互作用、基因组的重编程机制上进一步研究,同时也在技术上不断改进。克隆技术有广阔的应用前景,同时也存在着不少问题,基于此提出以胞质转移为基础的“逆向克隆技术”。结论:线粒体对卵母细胞的受精和胚胎发育有显著影响,但卵胞质移植可能导致线粒体的异质性及其潜在的问题还需要进一步研究。以卵胞质转移为基础的“逆向克隆技术”是否能达到克隆动物的结果前景喜人。OBJECTIVE： Inter-oocyte cytoplasmic transfer has been performed recently as a means to improve the outcome of assisted reproductive methods and becomes a hotspot. To overview the recent advances and problems in this area in this article, and put forth a new concept of reverse cloning technique based on ooplasmic transfer. DATA SOURCES： An online search of PUBMED was undertaken to identify correlative English articles published from January 1998 to December 2006 by using the keywords of ＂ooplasmic transfer, mitochondria heteroplasmy,animal cloning＂. Meanwhile, relevant Chinese articles published between January 1998 and December 2005 were searched in Wanfang database with the same keywords in Chinese. STUDY SELECTION：The articles ware primarily screened, and the citations at the end of each reference were reviewed. Inclusion criteria： The articles related to the research of ooplasmic transfer, cloning or reverse cloning. Exclusion criteria： Repetitive studies or Meta analytical articles. DATA EXTRACTION：Totally 126 relevant articles were collected, and 31 of them were in accordance with the inclusion criteria, while 95 of them were excluded because of repetition or obsolescence. In 31 articles met the criteria, 17 studies were relevant to the ooplasmic transfer, 13 studies related to animal cloning and one study related to reverse cloning. DATA SYNTHESIS： Exciting advances have been achieved in human gamete biology, and ooplasmic transfer have been widely applied. Many studies indicated that mitochondria in oocytoplasm affected obviously the fertilization of the oocytes and early embryo development. Lots of experiments have been done on the interaction of cytoplasm and nucleus as well as the mechanism of genomic reprogramming to further the investigation. Meanwhile, the techniques are progressed. Cloning techniques is excellent in application prospect, while there are some problems at the same time. Therefore the concept of reverse cloning technique was proposed based on ooplasmic transfer. CONCL

关 键 词：线粒体 遗传异质性 胚胎发育 克隆 生物 综述文献 

分 类 号：R711.74[医药卫生—妇产科学]