1种新的天然α-葡萄糖苷酶抑制剂的分离纯化及其活性测定  

Purification and activity of a new natural a-glucosidase inhibitor

作　　者：赵元[1] 张莲英[1] 胡晓燕[1] 崔福爱[1] 吴伟芳[1] 王晓蕾[1] 孙道旭[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012

出　　处：《中国生化药物杂志》2007年第1期20-22,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的从中草药中分离并得到α-葡萄糖苷酶抑制剂。方法中草药地榆通过水提、脱色、离心、离子交换色谱得到地榆多糖。用4-硝基酚-2-D吡喃葡萄糖苷(PNPG)法测定其对不同来源的α-葡萄糖苷酶活性的抑制率。结果地榆多糖显著抑制α-葡萄糖苷酶活性,并且可以降低大鼠餐后血糖浓度。结论地榆多糖是1种新的α-葡萄糖苷酶抑制剂。Purpose To find a a new natrul α-glucosidase Inhibitor from Sanguisorba officinalis L. Meth-ods Sanguisorba officinalis L. polysaccharide were prepared by soaking and decoloration centrifugation and chromatography .In vitro activity was assayed by using the PNPG substrae. In vivo, the Sanguisorba officinalis L. polysaccharide were given to SD rats and the effect on blood glucose level was observed. Results Sanguisorba officinalis L. polysacharide showed significant α-glucosidase inhibitory and hypoglycemic effect. Conclusion Sanguisorba offtcinalis L. polysacharide is a new natrul α-glucosidase Inhibitor.

关 键 词：地榆 多糖 Α-葡萄糖苷酶 抑制剂 

分 类 号：R284.2[医药卫生—中药学]