抗人膀胱癌单克隆抗体BDI-1的扩增和鉴定  

The production and characterization of antihuman bladder carcinoma monoclonal antibody BDI-1

作　　者：潘雪娜[1] 叶炯贤[2] 贾少微[1] 高凤彤[3] 

机构地区：[1]北京大学深圳医院核医学科,广东深圳518036 [2]北京大学深圳医院泌尿外科 [3]吉林大学中日联谊医院核医学科

出　　处：《中国实验诊断学》2007年第2期146-148,共3页Chinese Journal of Laboratory Diagnosis

基　　金：深圳市科技计划资助项目(200204116)

摘　　要：目的制备纯化的抗人膀胱癌单克隆抗体BDI-1。方法给Balb/c小鼠腹腔注射BDI-1杂交瘤细胞,收集的腹水过Protein G-agaros亲和层析柱即得到人膀胱癌BDI-1单克隆抗体。采用ELISA法、间接免疫荧光法、SDS-聚丙烯酰胺凝胶电泳法和点印迹法鉴定单克隆抗体。结果每毫升腹水可以得到1-1.5mg的高效价(106)的纯化抗体。结论该方法可以扩增高效价的单克隆抗体.To make use of purified antihuman bladder carcinoma monoclonal antibody BDI-1. Balb/c mice was injected hybridoma BDI-1 in peritoneal cavity,then use Protein C-agros affinity chromatography rarefied ascitesthat had been got.So we gained anfihuman bladder carcinoma monoclonal antibody BDI-1. Methods antibody was characterized by ELISA , indirect immunofluoresence ,SDS-PAGE electrophoresis and dot blot.Result; It was got 1-1.5 mg high titer（106） and purified antibody every milliliter ascites. Conclusion This way may produce high titer monoclonal antibody.

关 键 词：膀胱癌 BDI-1 单克隆抗体 

分 类 号：R737.14[医药卫生—肿瘤]