代谢木糖和葡萄糖的重组酿酒酵母的构建  

Construction of recombinant yeast metabolizing xylose and glucose

作　　者：刘继开[1] 田沈[1] 张亚珍[1] 张金鑫[1] 杨秀山[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《可再生能源》2007年第1期13-16,共4页Renewable Energy Resources

基　　金：国家863计划资助项目(2002AA514010;2001AA514024)

摘　　要：采用RT-PCR的方法克隆得到Candida shehatae木糖还原酶基因xyl1,将该基因插入到含有强启动子ADH1的穿梭质粒pACT2中,用同样的方法克隆得到Pichia stipitis的木糖醇脱氢酶基因xyl2,将该基因插入到含有强启动子PMA1的穿梭质粒pDR195中,两套外源基因连同各自的启动子和终止子将连接到酵母整合质粒YIp5-kanR中,并预期整合到酿酒酵母的基因组当中,从而构建代谢木糖和葡萄糖的重组酿酒酵母。Both Candida shehatae xyll gene and Pichia stipitis xyl2 gene were amplified by RT- PCR. The xyl1 and xyl2 genes were placed under the constitutive strong promoter ADH1 and PMA1 respectively of vector pACT2 and pDR195. In order to produce the integration vector, both genes along with their constitutive strong promoters will ligate into integration vector YIp5-kanR, establish a strain of recombinant yeast by transformation afterwards.

关 键 词：重组酵母菌 乙醇 木糖 染色体整合 木糖还原酶基因 木糖醇脱氢酶基因 

分 类 号：Q291[生物学—细胞生物学] Q933