降解偶氮染料耐盐菌GTY的分离鉴定及特性研究  

Isolation, identification and the growing condition of a dye-decolorization and salt-tolerant bacteria strain GTY

作　　者：郭建博[1,2] 周集体[1] 王栋[1] 田存萍[1] 王平[1] 李晓霞[3] 

机构地区：[1]大连理工大学环境与生命学院 [2]河北科技大学环境科学与工程学院,石家庄050018 [3]河北科技大学环境科学与工程学院

出　　处：《环境科学学报》2007年第2期201-205,共5页Acta Scientiae Circumstantiae

基　　金：辽宁省自然科学基金(No.20032122)~~

摘　　要：通过定向驯化，从海底污泥中分离得到一株降解偶氮染料的耐盐新菌株GTY。通过形态特征、生理生化特性以及对16SrDNA序列（GenBank Accession No. DQ286727）进行同源比较。鉴定泼菌株属于芽孢杆菌属（Gracilibacillus sp．，中国普通微生物菌种保藏中心CGMCC No.1528）．生长特性和染料降解实验结果表明，菌株对链霉素和卡那霉素不敏感，耐盐度为0．5％-30％．其最适降解与生长条件为：pH=7.8，温度30℃，盐度10％-20％．同时该菌株对13种不同结构的染料具有脱色作用。多数脱色率在90％以上。说明其具有较好的脱色广谱性和实际应用基础．A salt-tolerant strain capable to deeolorating azo dye was newly isolated from the coastal sediments contaminated with dye wastewater. It was identified as Gracilibacillus sp. based on morphological and physio-hioehemieal characteristics and 16S rDNA sequence analysis （GenBank Accession No, DQ286727） and was deposited in China General Microorganism Culture Center with the accession number CGMCC 1528, The results suggested that the strain could tolerant the antibiotics of kanamycin and streptomycin and the salt concentration range of 0. 5% - 30%. The optimal pH, temperature and the salt concentration for cell growth and K-2BP degradation were 7 - 8, 30℃ and 10% - 20% , respectively. The results of the strain decolorizing many dyes show that the strain could decolorize many dyes and be used in the treatment of dye wastewater in practice.

关 键 词：耐盐菌 偶氮染料 芽孢杆菌属 

分 类 号：X172[环境科学与工程—环境科学] X703