胞内信号转导分子LIM矿化蛋白-1在人牙周膜细胞体外矿化过程中表达的实验研究  

Expression of LIM mineralization protein-1 during mineralization of human periodontal ligament cells in vitro

作　　者：王效英[1] 张旗[1] 王强[2] 陈卓[1] 陈智[1] 

机构地区：[1]武汉大学口腔医学院教育部重点实验室,湖北武汉430079 [2]济南市口腔医院,山东济南250001

出　　处：《牙体牙髓牙周病学杂志》2007年第1期4-8,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30400499)

摘　　要：目的:研究胞内信号转导分子LIM矿化蛋白-1在人牙周膜细胞体外矿化过程中的表达。方法:利用组织块法体外培养人牙周膜细胞,实验组培养液中加入矿化诱导剂,对照组不加矿化诱导剂。培养14 d后,免疫细胞化学检测骨涎蛋白的表达;von Kossa染色检测矿化结节的形成。培养期间,采用半定量RT-PCR技术监测LIM矿化蛋白-1、碱性磷酸酶的表达,SPSS11.0软件分析结果。结果:培养14 d,实验组骨涎蛋白免疫细胞化学呈阳性表达,von Kossa染色阳性,矿化结节形成;对照组骨涎蛋白表达阴性,von Kossa染色阴性。RT-PCR结果显示碱性磷酸酶、LIM矿化蛋白-1在实验组和对照组各时段均表达。培养期间,实验组LIM矿化蛋白-1的表达显著高于对照组,其中培养第3 d、14 d时表达分别达到两个高峰,约为对照组的1.4倍、1.5倍。培养期间,碱性磷酸酶的表达显著增高,其中培养14 d时约为对照组的1.5倍。结论:首次发现LIM矿化蛋白-1与人牙周膜细胞的体外矿化过程相关,提示LIM矿化蛋白-1可能在牙周膜细胞的分化、矿化中发挥一定作用。AIM： To investigate the expression pattern of LIM mineralization protein - 1 during mineralization of cultured human periodontal ligament cells. METHODS： Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone, asorbic acid and β - glycerophosphate for bone - like mineralized nodule formation, characterized by yon Kossa staining and by immunocytochemistry of bone sialoprotein. Expression of LIM mineralization protein - 1 and alkaline phosphatase was analyzed by reverse transcriptase - polymerase chain reaction on day 0, 1,2, 3, 5, 7, 9, 11, 14, 21 and 28. RESULTS： During the mineralization, LIM mineralization protein - 1 and alkaline phosphatase were expressed at every time point, generally held at a statistically significantly higher level compared with the control. For LIM mineralization protein - 1, 1.4 - fold increased on day 3 and 1.5 - fold on day 14 ; for alkaline phosphatase, 1.5 -fold increased on day 14. CONCLUSION： LIM mineralization protein- 1 is found to be involved in the mineralization of human periodontal ligament cells. LIM mineralization protein - 1 may play a role in the differentiation and mineralization of human periodontal ligament cells.

关 键 词：牙周膜细胞 分化 矿化 LIM矿化蛋白-1 

分 类 号：R780.2[医药卫生—口腔医学]