结直肠癌、息肉及癌旁黏膜中二胺氧化酶表达的研究  

Expression of Diamine Oxidase in Colorectal Carcinomas,Polyps and Mucosa Adjacent to Colorectal Cancer

作　　者：汤菲[1] 单保恩[2] 陈兴[1] 张文[1] 

机构地区：[1]解放军白求恩国际和平医院,河北石家庄050082 [2]河北医科大学第四医院,河北石家庄050011

出　　处：《癌变．畸变．突变》2007年第1期19-21,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金："九五"全军医药卫生科研基金项目

摘　　要：背景与目的:制备抗人二胺氧化酶(DAO)单克隆抗体,分析结直肠组织中DAO的表达对结直肠肿瘤辅助诊断的价值和与临床病理参数之间的关系。材料与方法:应用免疫组织化学法检测不同结直肠病变组织中DAO的表达情况,比较其表达与结直肠肿瘤临床病理特征的关系。结果:DAO阳性定位于细胞浆。DAO在结直肠癌、腺瘤性息肉和癌旁黏膜组织中的表达率分别为80.0%、45.7%及3.39%,差别显著,并且在绒毛状腺瘤中的表达又显著高于管状腺瘤(P<0.05)。DAO在不典型增生结直肠组织中表达阳性,并随增生程度而递增。结论:DAO可能与结直肠肿瘤发生有关,是结直肠癌发生的早期异常生物标记,能较早预测肿瘤的发生。BACKGROUND ＆ AIM： We made anti-human diamine oxidase （DAO） monoclonal antibody, analyzed the expression of diamine oxidase in coloreetal tissue to assess its significance in pathologic diagnosis and its correlation with clinicopathologic parameters. MATERIALS AND METHODS： We used technique to study the expressions of DAO in different colorectal tissues.The expression was compared with clinicopathological features of colorectal neoplasia. RESULTS： DAO positive expression was found in cell cytoplasm. There were significant differences among the colorectal carcinomas, tous adenomatous polyps and mucosa adjacent to cancer. DAO positive expression rate in villous adenoma was significantly higher than that of tubulo adenoma（P 〈 0.05）. DAO was expressed in dysplatic tissues, and frequency increased with the progression of the dysplasia. CONCLUSION： DAO may be associated with the growth of colorectal neoplasia. It could be an early biomarker in human colorectal carcinomas and could predict growth of tumor at an early stage.

关 键 词：二胺氧化酶 结直肠肿瘤 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]