两种人类肝细胞系评价饮水氯化消毒副产物MX致DNA损伤作用的敏感性  

Susceptibility of Two Human-Derived Hepatic Cell Lines in Evaluating DNA Damage Induced by MX

作　　者：吴建军[1] 刘爱林[2] 邹亚玲[2] 周利红[2] 鲁文清[2] 

机构地区：[1]广州医学院化学致癌研究所,广州510182 [2]华中科技大学同济医学院公共卫生学院环境卫生教研室,湖北武汉430030

出　　处：《癌变．畸变．突变》2007年第1期33-35,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30170794)

摘　　要：背景与目的:研究两种人类来源的肝细胞在评价饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2(5氯)-呋喃酮(MX)DNA损伤作用的敏感性。材料与方法:应用单细胞凝胶电泳试验(彗星试验),研究人肝癌细胞株HepG2和人胚肝细胞L-02在评价饮水氯化消毒副产物MXDNA损伤作用的敏感性。结果:随着MX剂量增加,可引起HepG2(>30μmol/L)和L-02细胞(>100μmol/L)DNA迁移度显著增加;中度以上损伤所占百分比在同剂量组中HepG2细胞大于L-02细胞,经比较分析,差异有统计学意义(χ2=20.985;8.0;11.97,P<0.05)。结论:应用单细胞凝胶电泳试验评价MX的DNA损伤作用,人肝癌细胞株HepG2比人胚肝细胞L-02更为敏感。BACKGROUND ＆ AIM： To study on the susceptibility of two humn-derived hepatic cell lines in evaluating DNA damage induced by 3-chloro-4-dichloromethyl-5-hydroxy-2（5H）-furanone（MX） . MATERIALS AND METHODS： Using single cell gel electrophoresis assay, to evaluate the DNA damage induced by MX in human hepatocellular carcinoma cell lines（HepG2） and human fetal hepatocytes （L-02） . RESULTS： MX could induce HepG2 and L-02 DNA single strand breaks at the concentrations of 30 and 100μmol/L, respectively. The length of DNA migration induced by MX increased significantly in comparison to solvent control （DMSO） in a dose-response manner（P 〈0.05）. Moderate or greater DNA damages induced by MX in HepG2 were more serious than that of L-02（χ^2= 20.985; 8.0; 11.97, P 〈0.05）. CONCLUSION： HepG2 was more susceptible than L-02 cells to DNA damage induced by MX evaluated by using single cell gel electrophoresis assay.

关 键 词：HEPG2 L-02 单细胞凝胶电泳试验 DNA损伤 

分 类 号：R394.6[医药卫生—医学遗传学] R994.6[医药卫生—基础医学]