Bcl-2、Bcl-xL和Bax蛋白在结直肠癌中的表达  

The Expression of Bcl-2,Bcl-xL and Bax Protein in Colorectal Carcinomas

作　　者：李玉莉[1] 胡静姿[1] 刘惠敏[1] 余宏宇[1] 何金[1] 朱维健[1] 

机构地区：[1]第二军医大学长征医院病理科,上海200003

出　　处：《癌变．畸变．突变》2007年第1期53-55,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:通过检测结直肠癌与癌旁组织中Bcl-2、Bcl-xL和Bax蛋白的表达,以探讨其在结直肠癌发生发展中的作用。材料与方法:应用免疫组化S-P法检测39例结直肠癌组织与癌旁组织中Bcl-2、Bcl-xL和Bax蛋白表达的情况。结果:Bcl-2、Bcl-xL和Bax的阳性颗粒均主要分布于细胞质/膜,在癌旁组织中,Bcl-2阳性颗粒主要分布于陷窝的底部,Bax主要分布在靠近表面的上皮细胞,Bcl-xL则分布于整个陷窝。Bcl-2蛋白的表达在癌组织和癌旁组织都很低,两者之间差异无统计学意义(Z=0.072,P>0.05);Bcl-xL蛋白的表达均较高,且癌组织高于癌旁组织(Z=3.157,P<0.05);Bax蛋白的表达最强,但在癌组织和癌旁组织之间差异无统计学意义(P>0.05);只有Bcl-2蛋白表达与Dukes分期和TNM分期均成负相关(分别为rs=-0.389,-0.396,P<0.05),Bcl-xL和Bax蛋白表达与临床病理相关因素(性别、年龄、肿瘤大小、发生部位、组织学类型、分化程度、Dukes分期及TNM分期)均无关(P>0.05)。结论:Bcl-2可能只在结直肠癌发生发展的早期起重要作用,而后期可能Bcl-xL起更主要的作用,而Bcl-2蛋白表达可能会与预后有关。BACKGROUND ＆ AIM： To investigate the role of Bcl-2,Bcl-xL and Bax protein in colorectal carcinomatous kanorigenesis by detecting their expression in cancerous and adjacent tissues（ 〉 5cm from the cancerous tissue） MATERIALS AND METHODS： The expression of Bcl-2, Bcl-xL and Bax protein by S-P staining were detected in 39 colorectal cancerous tissues and adjacent tissues. RESULTS： The expression of Bcl-2 protein were often absent or weak, and no significant difference between cancerous tissue and adjacent tissue was found（Z= 0.072, P 〉0.05）.On the contrary, the immunostaining intensity of Bcl-xL protein was higher and stronger in cancerous tissues than adjacent tissues（Z = 3. 157, P 〈 0.05）, The immunostaining intensity of Bax protein was the strongest, but showed no significant difference between cancerous and adjacent tissues（Z= 1.707, P〉 0.05） Only the expression of Bcl-2 protein demonstrated a negative correlation with Dukes and TNM stages（ra=- 0.389, -0.396, P〈0.05）, all others had no relationship with clinicopathological parameters（P〉 0.05） . CONCLUSION： Bcl-2 gene might play an important role in the early stage of colorectal carcinomas,however, Bcl-xL gene might be more important in the later stage. On the other hand, Bcl-2 protein expression may correlate with prognosis.

关 键 词：结直肠癌 BCL-2 BCL-XL BAX 免疫组织化学 细胞凋亡 

分 类 号：R735.34[医药卫生—肿瘤]