牛磺酸对大鼠胸主动脉的舒张作用及其机制的研究  

Vasodilative effect and mechanism of taurine on thoracic aorta of rats

作　　者：薛文鑫[1] 张明升[1] 牛龙刚[1] 刘宇[1] 梁月琴[1] 

机构地区：[1]山西医科大学药理学教研室,山西太原030001

出　　处：《中国药理学与毒理学杂志》2007年第1期23-27,共5页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的 研究牛磺酸舒血管作用的可能机制。方法 记录苯肾上腺素（PE）和KCl预收缩的离体大鼠主动脉环张力变化，观察牛磺酸的舒血管作用及不同工具药对其作用的影响。结果 牛磺酸（20～80mmol·L^-1）对PE（1μmol·L^-1）或KCl（60mmol·L^-1）预收缩的大鼠主动脉环均有非内皮依赖的、浓度依赖性的舒张作用。在内皮完整的血管环，左旋硝基精氨酸甲酯（0．1mmol·L^-1）对牛磺酸的舒血管作用无明显影响；β-丙氨酸（60mmol·L^-1）在PE预收缩的血管环增强牛磺酸的舒血管作用，而在KCl预收缩的血管环则降低牛磺酸的舒血管作用；在KCl预收缩基础上，钾通道阻断剂格列本脲（10μmol·L^-1）和四乙胺（10mmol·L^-1）明显抑制牛磺酸的舒血管作用，而4-氨基吡啶（1mmol·L^-1）和BaCl2（1mmol·L^-1）无影响。结论 牛磺酸有浓度依赖性的血管舒张作用，此作用不依赖血管内皮，可能与其跨细胞膜转运有关，可能有钙依赖性钾通道和ATP敏感性钾通道的参与。AIM To investigate the vasodilative roles and the possible mechanisms of taurine on thoracic aorta of rats. METHODS Isotonic tension of thoracic aortic rings precontracted by phenylephrine （ PE, 1 μmol .L^-1 ） or KCl （60 mmol.L^-1） was recorded. The vasorelaxing action of taurine and the influence of various drugs on it were observed in the rings with endothelium intact or endothelium denuded. RESULTS Taurine （ 20 - 80 mmol . L^-1） caused concentration-dependent relaxation in thoracic aortas with or without endothelium, and there was no significant difference between them. NG-nitro-L-arginine methyl ester （0.1 mmol.L^-1） had no effect on the vasorelaxing action of taurine on thoracic aortas precontracted by PE or KCl. β-Alanine （60 mmol. L^- 1 ） diminished the vasorelaxing action of taurine in KCl-precontracted rings, but enhanced the action in PE-precontracted rings. Tetraethylamine, an antagonist of calcium activated potassium channels （ KBCa ） , and glibenclamide,an antagonist of ATP sensitive potassium channels （ KATP ） attenuated the vasorelaxing effect of taurine, but 4-aminopyridine and BaCl2 had no significant effect on the vasorelaxing action of taurine. CONCLUSION The vasorelaxing action of taurine is endothelium-independent and associated with taurine transmembrane transportation; KBCa and KATP may be involved in the action of taurine.

关 键 词：牛磺酸 主动脉 胸 血管舒张 丙氨酸 硝基精氨酸 钾通道阻滞剂 

分 类 号：R965[医药卫生—药理学]