吸烟和非吸烟者口腔良恶性肿瘤组织中P53蛋白的表达  

Expression of P53 protein in oral tumor tissue in smoking and nonsmoking subjects

作　　者：肖灿[1] 周建华[2] 惠建华[1] 李卫东[1] 

机构地区：[1]苏州大学附属第一人民医院,215006 [2]苏州大学放射医学与公共卫生学院

出　　处：《实用口腔医学杂志》2007年第1期15-18,共4页Journal of Practical Stomatology

基　　金：苏州大学青年教师基金资助(编号:Q3122402)

摘　　要：目的:研究吸烟与口腔肿瘤组织中P53蛋白表达的关系,探讨吸烟致口腔癌的发病机制。方法:选择22例吸烟及32例非吸烟口腔良恶性肿瘤患者的手术切除标本,良性病变切除后其边缘正常组织设为对照组。应用免疫组化技术SP法结合高温高压组织抗原修复,对P53蛋白表达进行检测,按组织病理类型进行分析。实验所得数据由SAS软件进行统计检验。结果:吸烟组P53阳性表达率为90.91%,非吸烟组为46.88%,2组阳性表达率有显著性差异(P<0.01)。恶性肿瘤的阳性表达率明显高于良性肿瘤和正常组织(P<0.01),良性肿瘤与正常组织的阳性表达率比较无差异(P>0.05),恶性肿瘤中,吸烟者P53表达的染色强度及阳性细胞数均明显高于非吸烟者。结论:吸烟对p53基因可产生损害性作用,烟草的刺激可使p53基因发生突变,这可能是香烟致癌的重要机制。本研究结果表明吸烟是诱发人体遗传物质损伤的重要诱变因素之一。Objectlve：To study the relationship between smoking and P53 protein expression in oral tumor tissue. Methods：The expression of P53 protein in oral tumor tissue was detected in 22 smoking cases and 32 nonsmoking cases by immunohistochemical SP method. SAS software was used for statistic analysis. Results：The positive expression rate of P53 protein in smokers （90.91%） was higher than that in nonsmokers （46.88%, P 〈 0.01 ）. There was a higher positive rate of P53 protein in malignant tumors （ 100% and 84.62% in smokers and nonsmokers respectively） than that in benign tumors and normal tissues（85.71% and 25.00% ,P 〈0. 01 ）. There was no difference of the expression between benign tumors and normal tissues （ P 〉 0.05 ）. Conclusion：Smoking may stimulate the mutation of p53 gene and play an important role in the carcinogenesis of oral tissues.

关 键 词：吸烟 口腔肿瘤 P53基因 

分 类 号：R739.8[医药卫生—肿瘤]