人宫颈上皮内瘤变和浸润癌组织中树突状细胞及T细胞的免疫组织化学定量分析  

IMMUNOHISTOCHEMICAL QUANTITY ANALYSIS OF DENDRITIC CELLS AND T LYMPHOCYTES IN HUMAN CERVICAL INTRAEPITHELIAL NEOPLASIA AND INVASIVE CARCINOMA

作　　者：常昕莹[1] 唐军民[1] 诸雪峻[2] 唐岩[1] 罗天霞[2] 李枫[1] 

机构地区：[1]北京大学基础医学院组织学与胚胎学教研室,北京100083 [2]北京市第六医院妇产科,北京100006

出　　处：《解剖学报》2007年第1期75-80,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(39970380)

摘　　要：目的研究人宫颈上皮内瘤变和浸润癌组织中树突状细胞和T细胞的数量、分布变化,为人宫颈癌的诊断和生物治疗提供实验依据。方法采用免疫组织化学和图像分析技术,测定人宫颈上皮内瘤变、宫颈浸润癌和正常宫颈组织中树突状细胞表型抗原CD1a、S-100及T细胞表面分化抗原CD3、CD8阳性细胞的数量、分布和表达强度。结果与人正常宫颈组织比较,宫颈上皮内瘤变中CD1+a、S-100+、CD3+、CD8+细胞数量增多(P<0.05),与组织分级呈正相关(P<0.05),抗体表达强度增高;宫颈浸润癌中4种抗体阳性细胞数量减少,与临床分期呈负相关(P<0.05),强度略有下降。结论人宫颈上皮癌前病变至浸润癌变过程中树突状细胞和T细胞数量及抗体表达强度发生变化,提示宫颈病变局部的免疫功能发生了改变。Objective To investigate the number, distribution and expression intensity of dendritic cells and T lymphocytes in human cervical intraepithelial neoplasia and invasive carcinoma for seeking biotherapeutic evidence of cervical carcinoma. Methods The number, distribution and-expression intensity of CD1a, S-100, CD3, CD8 positive cells were determined in cervical intraepithelial neoplasia and invasive carcinoma and human normal cervix tissue as the control, by using immunohistochemical technique and computerized imaging analysis system. Results Compared with human normal cervical tissue, the number and the expression intensity of CD1a^＋, S-100^＋ , CD3^＋ , CD8^＋ cells in cervical intraepithelial neoplasia significantly increased （ P 〈 0.05 ） and had a positive relation to histological grade （ P 〈 0.05 ） ; the number and the expression intensity of four antibody positive cells in invasive carcinoma decreased and had a negative relation to the clinical stage （ P 〈 0.05） .Conclusion The number and the expression intensity of dendritic cells and T lymphocytes changed in the process of cervical carcinogenesis, and the results suggest that there are some changes in local immunologic function in human pathological cervix.

关 键 词：宫颈上皮内瘤变 宫颈癌 树突状细胞 T细胞 免疫组织化学定量分析 人 

分 类 号：R730.21[医药卫生—肿瘤]