TGF-β_2反义寡核苷酸抑制抗青光眼术后滤过泡瘢痕的实验研究(英文)  

Experimental study of TGF-β_2 antisense oligodeoxynucleotide as an anti-scarring agent in glaucoma surgery

作　　者：李金瑛[1] 傅培[1] 杨琦[1] 

机构地区：[1]北京大学深圳医院眼科,中国广东省深圳市518036

出　　处：《国际眼科杂志》2007年第1期10-14,共5页International Eye Science

基　　金：中国广东省深圳市科技局资助项目(No.JH200505300483B)~~

摘　　要：目的:观察TGF-β2反义寡核苷酸对青光眼滤过术后结膜囊成纤维细胞的转化、增生的影响。方法:制备兔青光眼滤过手术模型。右眼结膜下注射TGF-β2反义寡核苷酸(A组);左眼分别注射TGF-β2错义寡核苷酸(B组)、PBS(C组),于造模后4,7,14,28d取术区结膜囊标本进行免疫组织化学染色和电镜观察。结果:A组眼压术后14d和21d比B组(P<0.01)和C组P<0.05)显著降低;滤泡平均生存时间A组(17.2d)较B组(14.5d)、C组(13.5d)明显延长(P<0.05);A组α-SMA和PCNA阳性成纤维细胞数明显少于B、C组;成纤维细胞的超微结构A组和B、C组比较无明显区别。结论:TGF-β2反义寡核苷酸可降低兔青光眼滤过术后眼压,延长滤泡生存时间,抑制兔青光眼滤过术后结膜囊成纤维细胞的转化、增生。AIM： Currently available anti-scarring regimens for glaucoma filtration surgery have potentially blinding complications and safer alternatives would be beneficial. This experiment is to investigate the effect of TGF-β2 antisense oligodeoxynucleotide on differentiation, proliferation of subconjunctival fibroblast following glaucoma filtration surgery. METHODS： Glaucoma filtration surgery were performed on both eyes of 28 rabbits. TGF-β2 antisense oligodeoxynucleotide was subconjunctivally injected in the right eyes （A group）, and TGF-β2 missense oligodeoxynucleotide （B group） or PBS（C group） was used at the same method in the left eyes as controls. Rabbits were killed at 4,7,14 and 28 days after surgery. Intraocular pressure （IOP）, bleb characteristics were recorded at different time point. Subconjunctival fibroblasts were examined by immunohistochemistry and electron microscopy. RESULTS： The IOP of rabbits in group A was significantly lower at 14 days （6.74± 1.18 mmHg） and 21 days （8.15± 1.97 mmHg） after operation than the IOP in group B （8.53± 1.04, 9.72± 1.09mmHg）（P 〈0.01） and group C（8.79± 1.21, 9.43± 1.27mmHg） （P 〈0.05）. The mean bleb survival time was longer （17.2 days） in group A than that of group B （14.5 days） and group C （13.5 days）（P〈0.05）. The population of the cells expressing α-smooth muscle actin（α -SMA） and proliferating call nuclear antigen （PCNA） was significantly reduced in group A compared with the group B and C. The ultrastructure of fibroblast was not altered by TGF-β2 anti-sense oligodeoxynudeotide. CONCLUSION：TGF-β2 antisansa oligodeoxynudeotide can prevent the scar formation after glaucoma surgery by inhibit the differentiation and proliferation of subconjunctival fibroblast. It could be a potentially useful anti-scarring alternative for the prevention of late surgical failure.

关 键 词：TGF-Β2 反义基因治疗 青光眼 瘢痕 

分 类 号：R779.6[医药卫生—眼科]