Ets-1在链脲左菌素诱导的糖尿病大鼠模型视网膜内表达增强(英文)  

Increased expression of Ets-1 in the diabetic retina of streptozotocin-induced rat model

作　　者：杜兆江[1] 李鹏[2] 惠延年[1] 王百忍[3] 段小莉[3] 张瑞[4] 

机构地区：[1]第四军医大学西京医院眼科,中国陕西省西安市710032 [2]解放军第451医院眼科,中国陕西省西安市710054 [3]第四军医大学全军神经生物研究所,中国陕西省西安市710032 [4]第四军医大学唐都医院药剂科,中国陕西省西安市710038

出　　处：《国际眼科杂志》2007年第1期19-22,共4页International Eye Science

摘　　要：目的:阐明Ets-1是否参与了实验性糖尿病视网膜病变过程。方法:腹腔注射链脲左菌素(STZ)来诱导糖尿病。STZ注射后4wk,分离各组视网膜内总RNA和总蛋白,分别进行Northern blot和Western blot分析。结果:实验性糖尿病视网膜内Ets-1在mRNA水平和蛋白水平均明显高于对照组(P<0.001)。结论:实验结果表明Ets-1参与了实验性糖尿病视网膜的病理过程。进一步研究需要阐明Ets-1和VEGF关系。AIM： To determine the involvement of Ets-1 in the pathological progress of the experimental diabetic retina. METHODS： Diabetes was induced by intraperitoneal injection of STZ. Total RNA and Total proteins were isolated from retinas of experimental and control eyes at 4 weeks after STZ-injection and were assessed by Northern blot analysis and Western blot analysis, respectively. RESULTS： Expression of both Ets-1 mRNA and Ets-1 protein was significantly increased in the experimental diabetic rat＇s retina after STZ-injection compared with the control group （P〈0.001）. CONCLUSION： Our results indicated that Ets-1 was involved in the pathological progress of experimental diabetic retina. Further studies should be conducted to focus on the relationship between Ets-1 and VEGF in the diabetic retina.

关 键 词：ETS-1 VEGF 糖尿病视网膜 新生血管 

分 类 号：R774.1[医药卫生—眼科]