胃癌组织中p53基因突变及蛋白表达的研究  

The study of p53 gene mutation and p53 protein expression in gastric carcinoma

作　　者：于燕妮[1] 陈杨[1] 胡军[1] 方艺[1] 樊毓[1] 

机构地区：[1]贵阳医学院病理教研室,550004

出　　处：《贵州医药》2007年第2期107-109,共3页Guizhou Medical Journal

基　　金：贵州省省长基金资助项目(S2004-10)

摘　　要：目的探讨胃癌组织中p53基因第5～8外显子突变率及p53蛋白的表达。方法应用聚合酶链反应-单链构象多态性分析(PCR-SSCP)和银染技术,对30例胃癌石蜡包埋组织中p53基因第5～8外显子突变率进行检测;同时应用免疫组织化学Envision二步法检测胃癌组织中p53的表达水平。结果30例胃癌石蜡标本中有11例发生p53基因的突变,突变率为36.67%。病理组织学观察胃癌中乳头状腺癌/管状腺癌(分化较好)16例,低分化腺癌/印戒细胞癌(分化较差)14例。p53蛋白随胃癌的分化程度降低而增加,阳性表达率递增(P<0.05)。结论胃癌组织中p53基因突变及p53蛋白高表达在胃癌的发生中有重要的作用及意义。从石蜡包埋组织中提取DNA进行分子生物学检测适用于肿瘤研究。Objective To investigate the p53 gene mutation rate in exons 5-8 and the expression of p53 protein in gastric carcinoma. Methods PCR-SSCP silver staining was used to test the mutation in the 5,6,7,8 exons of p53 gene and the Envision immunohistochemical method was applied to the expression of p53 in 30 cases of gastric carcinoma in paraffin-embedded tissues. Results Mutation was found in 11 cases out of 30 cases of gastric carcinoma in paraffin-embedded tissues （36.67%）. The positive expression rate of p53 was much higher in the poor differentiation in gastric carcinoma （P〈 0. 05）. Conclusion The p53 gene mutation play an important role in gastric carcinoma.

关 键 词：胃癌 P53基因 聚合酶链反应-单链构象多态性分析 第5~8外显子突变 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]