10种多孔类真菌体外抗肿瘤活性的筛选  

Screening of Anti-tumor Activity from Ten Polypores Fungi in vitro

作　　者：孙勇[1] 蒋继宏[1] 陈玉芹[1] 高甜惠[1] 陈凤美[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点试验室,徐州221116

出　　处：《微生物学通报》2007年第1期112-115,共4页Microbiology China

基　　金：江苏省药用植物生物技术重点实验室开放课题(No.ZXL0512)

摘　　要：采用MTT法对10种多孔类真菌的发酵液和菌丝体提取物进行抗人肺癌细胞实验,测定培养基种类和培养时间对菌株抗肿瘤活性的影响。结果表明:PDA培养基培养的Onnia tomentosa和Ceizene unicolon的发酵液以及木屑马铃薯培养基培养的Formitopsis pinicola菌丝醇提物具有显著的抗肿瘤活性,其中Formitopsis pinicola菌丝醇提物浓度为500μg/mL时对肿瘤细胞的抑制率达到88．87%;且在不同的培养基中及不同的培养时间获得的菌丝醇提物的抗肿瘤活性变化不大。The anti-tumor activity of fermentation and the ethanol extraction of ten Polyprous fungi against human lung cancer cell NCI-H460 were determined by MTT method, the effect of medium and time for cultivation on the anti-tumor activity were studied. The results showed that the fermentation liquid of Onnia tomentosa and Ceizene unicolon and the mycelium ethanol extract of Formitopsis pinicola had obvious anti-tumor activity, the tumor inhibiting ratio of mycehum ethanol extract of Formitopsis pinicola cultivated in Potato-woedchipping medium was 88. 87% at the concentration for 500μg/mL, and the inhibiting ratio had little change when Formitopsis pinicola cultivated in different medium and for different days.

关 键 词：多孔菌 筛选 抗肿瘤活性 

分 类 号：R730.5[医药卫生—肿瘤]