涎腺腺样囊性癌与黏液表皮样癌中耐药、转移相关基因表达的比较研究  

Expression of drug resistance and metastasis related gene in adenoid cystic carcinoma and mucoepidermoid carcinoma

作　　者：王晓峰[1] 郭阳[1] 贾深汕[2] 

机构地区：[1]哈尔滨医科大学第二临床医学院口腔颌面外科,150086 [2]哈尔滨医科大学第三临床医学院头颈外科

出　　处：《中华口腔医学杂志》2007年第2期78-79,共2页Chinese Journal of Stomatology

基　　金：黑龙江省自然科学基金(C00C191201)

摘　　要：目的应用基因芯片对腺样囊性癌(adenoid cystic carcinoma,ACC)以及低分化来源的黏液表皮样癌(mucoepidermoid carcinoma,MEC-1)进行基因筛查,并对3种重要的基因进行实时定量PCR 检测,以便为临床早期诊断和合理治疗提供依据。方法应用基因芯片筛查,选出3个重要的基因,利用实时定量 PCR 进行检测。结果多药耐药基因(multidrug resistance gene,MDR)1含量 MEC-1>ACC-M>ACC-2;多药耐药蛋白(multidrug resistance protein,MRP)1含量 MEC-1>ACC-M>ACC-2;CCD1基因含量 MEC-1>ACC-M>ACC-2。结论 MEC-1耐药性与 MDR-1基因含量较高相关;ACC-M 高转移性与 MRP-1基因含量较高和 CCD1基因含量较低相关;ACC-2低转移性与 MRP1基因含量较少相关。Objective To examine the differential gene expression among adenoid cystic carcinoma ACC-M, low metastasis ACC-2 and low differentiated mucoepidermoid carcinoma MEC-1 with gene array, and detect three important genes with real-time quantitative PCR. Methods Gene array was used to screen the differential genes, then three important genes were selected and detected by real-time quantitative PCR. Results The gene contents of MDR-1, MRP-1 and CCD1 were MEC-1 〉 ACC-M 〉 ACC-2, MEC-1 〉 ACC-M 〉 ACC-2 and MEC-1 〉 ACC-M 〉 ACC-2 respectively. Conclusions Drug resistance of MEC-1 is related with high content of MDR-1. High metastasis of ACC-M is concerned with high content of MRP-1 and low content of CCD-1. Low metastasis of ACC-2 has a relationship with low content of MRP1.

关 键 词：腺样囊性癌 黏液表皮样癌 基因 

分 类 号：R739.8[医药卫生—肿瘤]