肝细胞癌中DcR3表达与癌细胞凋亡的关系研究  

Relationship between expression of decoy receptor 3 and apoptosis in hepatocellular carcinoma

作　　者：陈罡[1] 罗殿中[1] 王云[1] 廖芝玲[1] 张美艳[2] 

机构地区：[1]广西医科大学病理教研室/南宁广西医科大学第一附属医院病理科,530021 [2]桂林医学院病理学教研室

出　　处：《中华病理学杂志》2007年第2期113-117,共5页Chinese Journal of Pathology

基　　金：中国高等学校博士学科点专项科研基金(20050598005);广西科学研究与技术开发计划应用基础研究基金(桂科基0639040)

摘　　要：目的探讨诱捕受体3(decoy receptor 3,DcR3)蛋白在原发性肝细胞癌(HCC)中的表达及其与癌细胞凋亡和 HCC 预后的关系。方法应用免疫组织化学(EnVision 法)及和脱氧核糖核酸末端转移酶介导的缺口末端标记(TUNEL)技术检测43例 HCC,16例非癌肝组织(单纯性肝硬化及肝血管瘤周围正常肝组织)中 DcR3蛋白的表达及凋亡情况,并分析其与临床病理参数的关系。结果DcR3明确定位于肝细胞胞质内;HCC 组织中的 DcR3蛋白的阳性率为74.42%(32/43),明显高于非癌肝组织43.75%(7/16,P<0.05);HCC 中有转移癌组 DcR3阳性率为100%(22/22),明显高于无转移组52.94%(9/17,P<0.01);DcR3蛋白的表达与 AFP 水平(r=0.444,P<0.01)、门静脉癌栓(r=0.414,P<0.01)有关,与年龄、性别、有无肝硬化、包膜浸润、肿瘤结节数及分化程度无关(P>0.05)。HCC 中细胞凋亡指数[AI(0.78±0.64)%]明显低于非癌肝组织[(3.32±1.81)%,P<0.01];HCC 临床 TNM 分期Ⅰ、Ⅱ期 AI(1.03±0.69)%高于Ⅲ、Ⅳ期[(0.52±0.48)%,P<0.01];HCC 无转移组 AI(1.10±0.72)%高于转移组[(0.44±0.27)%,P<0.01];AI 与 AFP 水平(r=-0.468,P<0.01)、门静脉癌栓(r=-0.434,P<0.01)、包膜浸润(r=-0.331,P<0.05)有关,与年龄、性别、有无肝硬化、肿瘤结节数及分化程度无关(P>0.05)。在 HCC 和非癌肝组织中,DcR3阳性者的 AI 均分别低于阴性者的 AI(均 P<0.01)。结论 DcR3表达可影响凋亡并在 HCC 的发生发展中起重要作用;检测 DcR3蛋白与 AI 有助于判断 HCC 患者预后。Objective To study the expression of decoy receptor 3 （DcR3） and its relationship with apoptosis and prognosis in hepatocellular carcinoma （ HCC ）. Methods The expression of DcR3 protein in 43 cases of HCC and 16 cases of non-cancerous liver （ including cirrhotic liver tissue and normal liver tissue adjacent to cavernous hemangioma） was studied by immunohistochemistry （using EnVision method）. The status of apoptosis was evaluated by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） technique. Statistic analysis was carried out to assess the correlation between DcR3 expression, apoptotic index （AI） and clinicopathologic porameters. Results DcR3 protein was expressed in the cytoplasm of HCC cells. The positivity rate of DcR3 in HCC was 74. 42% （32/43） , which was significantly higher than that in the non-cancerous group （43.75% ,P 〈0. 05）. The positivity rate of DcR3 in HCC with metastasis detected within 20 months of diagnosis was 100% （ 22/22 ）. This was significantly higher than that in HCC without metastasis （ 52.94% , P 〈 0. 01 ）. The DcR3 expression in HCC also correlated with serum alpha-fetoprotein level （ r =0. 444, P 〈0. 01 ） and presence of tumor embolus in portal vein （ r = 0. 414, P 〈 0. 01 ）. However it had no relationship with the patient＇s age, sex, cirrhotic status, liver capsule invasion, number of tumor nodules and histologic differentiation （ P 〉 0. 05 ）. The AI in HCC （0. 78 ± 0. 64 ）% was significantly lower than that in the non-cancerous group [ （3. 32 ± 1.81 ）% , P 〈0. 01 ]. The AI in clinical TNM stage Ⅰ and Ⅱ tumors （ 1.03 ±0. 69）% was significantly higher than that in stage Ⅲ and Ⅳ tumors [ （ 0.52 ± 0. 48 ） %, P 〈 0. 01 ]. The AI in HCC without metastasis （ 1.10 ± 0. 72）% was significantly higher than that in HCC without metastasis [ （0. 44 ±0. 27）% ,P 〈0. 05]. The AI correlated with serum alpha-fetoprotein level （ r = - 0. 468, P 〈 0.01 ）, presence

关 键 词：癌 肝细胞 细胞凋亡 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]