甲基苯丙胺对相关脑区的神经毒性作用  

Neurotoxicity of methamphetamine on related brain regions in rats

作　　者：李学锋[1] 邱平明[1] 金贺[1] 刘明[1] 王慧君[1] 

机构地区：[1]南方医科大学基础医学院

出　　处：《解剖学杂志》2007年第1期63-65,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30572090)

摘　　要：目的:研究甲基苯丙胺(MA)对大鼠纹状体、海马、额叶皮质等脑区神经细胞的毒性作用以及对大鼠行为的影响。方法:H-E染色、Glees银浸染观察神经元和轴突的变化;高效液相色谱检测上述脑区多巴胺(DA)及其代谢产物含量;免疫组化胶质纤维酸性蛋白(GFAP)检测胶质细胞增生情况。结果:MA对上述脑区神经细胞和轴突有损伤作用,表现为神经细胞变圆,极性消失;胶质细胞增生,噬神经细胞现象、胶质小结形成;神经轴索扭曲,节段性增粗,轴索间隙增宽;GFAP阳性星形细胞增多;纹状体DA及其代谢产物含量显著降低,海马、额叶皮质DA含量明显降低;大鼠行为改变明显。结论:MA对大鼠中枢神经系统多脑区神经元有明显的毒性作用,可导致上述脑区DA含量下降和大鼠行为改变。Objective： To observe the neurotoxicity of methamphetarnine （MA） on the rat brain. Methods： Histological changes of the striatum, hippocampus and frontal cortex were observed by HE and Glees silver staining. Dopamine （DA） and dihydroxyphenylacetic acid （DOPAC） of those brain regions were detected by HPLC. GFAP was detected by immunohistochemistry. Results： Injuries of the neurons and axons were observed after MA treatment, such as swelling of the neurons, proliferation of the glial cells, neuronophagy, formation of glial nodule, snaky like and segmental enlargement of the axons and increased expression of GFAP. DA decreased in the striatum, hippocampus and frontal cortex, and I）OPAC in the striatum also decreased. The behavior of rats obviously changed after MA injection. Conclusion.. MA has obvious neurotoxicity on multiple regions of the rat brain, and can cause neuron injuries, DA depletion and behavioral changes.

关 键 词：甲基苯丙胺 神经毒性 行为变化 

分 类 号：R363[医药卫生—病理学]