广西金秀瑶族15个STR基因座遗传多态性  

Genetic polymorphisms of 15 STR loci of Jinxiu Yao ethnic group in Guangxi province

作　　者：李松峰[1] 徐林[1] 龚继春[1] 罗国容[1] 周丽宁[1] 邓琼英[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021

出　　处：《解剖学杂志》2007年第1期84-87,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30260044)

摘　　要：目的：了解广西金秀瑶族群体15个STR基因座的遗传多态性。方法：Chelex-100法提取样本DNA，荧光标记复合扩增基因座，ABIPrism3100型遗传分析仪对扩增产物进行检测。结果：15个位点共检测出113种等位基因，基因频率分布在0．0029～0．5514；366种基因型，频率分布在0．0054～0．3657之间；符合Hardy-Weinberg平衡定律；杂合度（H）分布在0．5718～0．9286，个人识别力（DP）分布在0．7241～0．9601，累积个人识别力TDP为〉0．9999999999，非父排除率（EP）在0．3658～0．8783，累积非父排除率CEP为0．999999998，多态信息量PIC分布在0．4953～0．8428。结论：广西金秀瑶族15个STR基因座除TPOX基因座外，其它基因座均属于高度多态性遗传标记。Objective： To study the STR genetic structure of Jinxiu Yao ethnic group in Guangxi province. Methods Distribution of 15 STR loci was determined by STR genescan marked by fluorescence. Results： Totally 113 alleles and 366 genotypes were observed in 175 unrelated Jinxiu Yao individuals, with the corresponding gene frequency and genotype frequency being 0. 002 9-0. 551 4 and o. 005 4-0. 365 7, respectively. The genotypes of 15 STR loci were in accordance with the Hardy-Weinberg equilibrium （P〉0. 05）. The statistical analysis of 15 STR loci showed PIC （polymorphism information content） was 0. 495 3-0. 842 8; H （heterozyaosity） , 0. 571 8-0. 928 6; DP （discrimination power） , 0. 724 1-0. 960 1; and EP （Excluding probability of paternity） , 0. 365 8-0. 878 3. According to the obtained data, the cumulative exclusion power was 0. 999 999 998 and the cumulative discrmination power was more than 0. 999 999 999 9. Conclusion： The results indicate the 15 STR loci （except TPOX） of Jinxiu Yao ethnic group in Guangxi province have high genetic polymorphism, playing an important role in Chinese population genetic study.

关 键 词：金秀瑶族 STR基因座 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学]