NaCl胁迫对马铃薯抗氧化系统的影响  

Effect of NaCl Stress on Antioxidant Defense System of Potato

作　　者：张瑞玖[1] 尚国斌 蒙美莲[1] 门福义[1] 郦海龙[1] 郭俊秀[1] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010019 [2]内蒙古武川县财政局,内蒙古武川011700

出　　处：《中国马铃薯》2007年第1期11-14,共4页Chinese Potato Journal

摘　　要：以紫花白、大西洋、克新12号3个马铃薯品种为试验材料,研究了NaCl胁迫下其体内过氧化物酶活性、超氧化物歧化酶活性和过氧化物酶同工酶的变化。结果表明:随着NaCl胁迫浓度的增加,马铃薯3个品种过氧化物酶活性均表现为随NaCl浓度的增加先下降后上升,而超氧化物歧化酶活性表现为随NaCl浓度的增加先降低,再升高,然后再降低的变化规律。但是,3个品种两种酶的变化幅度却不相同。克新12号在0.3%和0.45%NaCl浓度下两种酶的活性均迅速上升,且在0.45%浓度下显著的高于对照;紫花白在NaCl胁迫下两种酶活性变化较平稳,和对照差异不显著;大西洋在不同NaCl浓度下两种酶的活性均低于对照,且过氧化物酶活性在0.30%和0.45%的NaCl浓度下显著的低于对照;克新12号和紫花白在较高的NaCl浓度下过氧化物同工酶谱带较深,大西洋则随NaCl浓度的升高同工酶谱带的颜色变浅,同时,耐盐品种克新12号和紫花白在B区均诱导出了一条明亮的谱带。Three potato cuhivars, Zihuabai, Atlantic and Kexin 12, were used to do an experiment which monitored the changes in peroxides activity, superoxide dismutase activity and peroxidase isoenzyme under NaCl stress. With the increase of NaCl concentrations, the peroxidase activity of the three potato cultivars decreased at first, then increased, while superoxide dismutase activity declined at first, then increased and decreased finally. However, the range of changes of the two enzymes in the three cuhivars was different. When NaCl concentrations reached 0.30% and 0.45%, the two enzyme activity of Kexin 12 increased rapidly and was significantly higher under the concentration of 0.45% than that of the control; the two enzyme activity of Zihuabai changed little bit under NaCl stress, and had no significant difference under stress compared with controls; the two enzyme activity of Atlantic was lower than the control in different concentrations, and peroxidase activity was significantly lower than that of the control under the concentrations of 0.30% and 0.45%. The bahd color of peroxidase isoenzyme blackened in Zihuabai and Kexin 12 under higher concentrations of NaCl, whereas the band color of Atlantic lightened gradually with increase of NaCl cocentrations. In adition, Kexin 12 and Zihuabai was induced a bright band in region B.

关 键 词：马铃薯 NACL胁迫 过氧化物酶 超氧化物歧化酶 

分 类 号：S532[农业科学—作物学]