过氧化氢对PC12细胞中硫氧还蛋白和APE/Ref-1基因表达的影响  

Effect of hydrogen peroxide on the expression of thioredoxin and APE/ref-1 in PC12 cells

作　　者：谢振华[1] 刘长振[2] 王爱民[3] 马春[3] 

机构地区：[1]清华大学深圳研究生院生命科学与海洋生物学实验室,广东深圳518055 [2]清华大学生物科学与技术系 [3]哈尔滨医科大学生物化学和分子生物学教研室

出　　处：《中国老年学杂志》2007年第3期247-249,共3页Chinese Journal of Gerontology

摘　　要：目的观察过氧化氢(H2O2)对硫氧还蛋白和APE/Ref-1基因表达的影响,探讨H2O2预处理对神经细胞具有适应性保护的分子机制。方法以PC12细胞作为神经细胞模型,以200和400μmol/L浓度的H2O2处理PC12细胞,用MTT法检测细胞的损伤程度,以Western blot方法测定硫氧还蛋白和APE/Ref-1表达水平。结果200μmol/LH2O2促进PC12细胞的硫氧还蛋白和APE/Ref-1表达,400μmol/LH2O2也促进PC12细胞的APE/Ref-1表达,但使PC12细胞的硫氧还蛋白表达显著降低。结论促进硫氧还蛋白和APE/Ref-1表达可能是低浓度H2O2预处理对神经细胞具有适应性保护的分子机制。Objective To observe the effect of hydrogen peroxide （H2O2） on the expression of thioredoxin and APE/ref-1 and investigate the molecular mechanism of the adaptive protection of H2O2 preconditioning. Methods As neural cell model,PC12 cells were treated with 200 and 400 μmol/L of H2O2 ,and the injury of PC12 cells was assessed by MTT. The expression of thioredoxin and APE/ref-1 in PC12 cells was detected by Western blot. Results 200 μmol/L H2O2 enhanced the expression of thioredoxin and APE/ref-1 in PC12 cells, and 400 μmol/L H2O2 enhanced the expression of APE/ref-1 too. But 400 μmol/L H2O2 significantly decreased the expression of thioredoxin. Conclusions The high expression of thioredoxin and APE/ref-1 induced by low concentration H2O2 preconditioning may be a part of the molecular mechanism of the adaptive protection of H2O2 preconditioning.

关 键 词：PC12细胞 硫氧还蛋白APE/ref-1 过氧化氢 

分 类 号：R749.16[医药卫生—神经病学与精神病学]