转4-1BBL基因的小鼠肝癌细胞瘤苗刺激脾细胞产生细胞因子的研究  

Cytokines Production of Murine Spleen Cells Induced by Gene Transfer of 4-1BBL into Hepatocellular Carcinoma Hepa1-6 in Vitro

作　　者：刘承利[1] 臧晓霞[2] 窦科峰[3] 朱帮福[4] 张洪义[1] 张宏义[1] 

机构地区：[1]空军总医院肝胆外科,北京100036 [2]空军总医院口腔科,北京100036 [3]第四军医大学西京医院肝胆外科 [4]第四军医大学基础部生化教研室

出　　处：《肿瘤防治研究》2007年第2期115-117,共3页Cancer Research on Prevention and Treatment

摘　　要：目的研究转4-1BBL基因的小鼠肝癌细胞瘤苗体外刺激同系小鼠脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的能力。方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。结果TCV-4-1BBL刺激后,脾细胞体外分泌细胞因子IL-2、TNF-α和GM-CSF的水平明显增高。结论转4-1BBL基因的小鼠肝癌细胞瘤苗能刺激脾细胞产生细胞因子IL-2、TNF-α和GM-CSF。Objective To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepal-6. Methods Murine hepatocellular carcinoma cell line Hepal-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine （TCV-m4-1BBL） was obtained by treating Hepal-6-m4-1BBL with mitomycin （MMC）. Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines （IL-2, TNF-α and GM-CSF）. Results Comparing with TCV-Hepal-6, high levels of IL-2, TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL. Conclusion Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2, TNF-α and GM-CSF.

关 键 词：小鼠 4-1BBL 共刺激分子 肿瘤疫苗 肝细胞癌 细胞因子 

分 类 号：R735.7[医药卫生—肿瘤]