三氧化二砷抑制兔后发性白内障的实验研究  

Inhibitory effect of arsenic trioxide on posterior capsule opacification of rabbit eyes after operation

作　　者：关立南[1] 刘平[1] 李志坚[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院眼科医院,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2007年第1期32-35,共4页Journal of Harbin Medical University

基　　金：黑龙江省卫生厅资助项目(2005-141)

摘　　要：目的从研究抑制后发性白内障的有效药物入手,探讨术中水分离时应用三氧化二砷对兔术后晶体上皮细胞增殖的影响及其对兔眼的毒性作用。方法在兔晶状体囊外摘除术中,用1ml不同浓度的三氧化二砷(2μmol／L、4μmol／L、8μmol／L)在晶状体囊袋内进行水分离,使其直接短暂作用于晶状体上皮细胞。术后随访12周,进行术眼裂隙灯检查、观察眼内压变化及角膜内皮细胞差异,检测术后组织病理学及超微结构的变化,对三氧化二砷抑制兔术后晶体上皮细胞增殖效果进行分析。结果术后2周用药组兔眼未出现后囊膜混浊(posterior capsule opaeifica- tion,PCO)。术后4周用药组兔眼的PCO明显比对照组眼轻,且随药物浓度增高而减轻。中、低浓度组兔眼的术后炎症反应与对照组比较无明显差异;高浓度组兔眼术后早期出现轻微的毒性反应。组织病理学检查表明,对照组的晶状体上皮细胞增生较用药组明显。结论①在活体兔中,三氧化二砷有效地抑制晶状体上皮细胞的增殖,可延缓和防止PCO的形成;②三氧化二砷对晶体上皮细胞的抑制作用有浓度依赖性;③兔晶状体囊袋内应用2- 8μmol／L的三氧化二砷对角膜内皮细胞没有明显的毒性作用,是防治后发性白内障安全有效的剂量浓度。Objective To study the inhibitory effect of arsenic trioxide（As2O3） applied by hydrodissection method on posterior capsule opacification of rabbit lens and evaluate its intraocular toxicity.Methods lml of 2μmol/L,4μmol/L, and 8μmol/L As2O3 were injected into the capsular bags of the rabbits＇ lens during the hydrodissection of extracapsular cataract extraction, respectively. The drug was exposed to the lens epithelial cells directly and transiently. In the 12-week follow-up after the operation,the posterior capsule opacification, the change of intraocular pressure and the corneal endothelial cell were observed and compared between the control group and the experimental group; The histopathologic and the ultrastructural changes were also observed.Results The posterior capsule opacifications only appeared in the control group two weeks after the operation. Four weeks after the operation, the posterior capsular opacification in the experimental group was not mild than that in the control group. The higher the concentration of the drugs, the less the posterior capsule opacification was shown. There was no significant difference between the oostoperative inflammation in the control group and the low, middle-concentration experimental group. Slight toxic reaction was observed in the rabbits＇ eyes of the high-concentration group at early stage after the operation. The histopathologic examination showed that the proliferation of lens epithelial cells in the control group was more obvious than that in the experimental group. Conclusion The results showed that As2O3 can inhibit the proliferation of lens epithelial ceils effectively, significantly prevent the development of PCO in rabbit eyes. Its efficiency is related to the drug＇s concentration. The toxicity of As2O3 to the corneal endothelial cells is not observed, it＇s a safe and effective durg to prevent the after-cataract when As2O3 in concentrations of 2μmol/L are applied in the capsular bag.

关 键 词：三氧化二砷 后发性白内障 水分离 兔 

分 类 号：R-332[医药卫生] R776.1