白果内酯治疗大鼠卡氏肺孢子虫肺炎后的形态学变化  

Pathological changes in lungs of rats infected with Pneumocystis carinii pneumonia after treatment with bilobalide

作　　者：唐小葵[1] 倪小毅[2] 王健[2] 江涛[1] 

机构地区：[1]重庆医科大学附属第一医院肺科,重庆400016 [2]深圳市第二人民医院感染科,深圳518035

出　　处：《重庆医科大学学报》2007年第3期243-246,302,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助(30070680)。

摘　　要：目的:研究经白果内酯治疗后卡氏肺孢子虫肺炎(PCP)大鼠的肺部病理学变化。方法:以地塞米松连续6周皮下注射Wistar大鼠,建立卡氏肺孢子虫肺炎动物模型,腹腔内注射白果内酯30mg/(kg.d)×8天,停药1周后杀鼠取肺,光镜和电镜观察大鼠肺部病理学变化,同时设有感染对照组和正常对照组。结果:经白果内酯治疗后肺印片中卡氏肺孢子虫(Pc)包囊数显著减少,肺部炎症明显吸收。透射电镜观察Ⅰ型肺泡上皮细胞水肿明显减轻,肺孢子虫滋养体大量空泡形成、细胞器肿胀破坏,髓样结构形成、胞膜破坏和胞质内出现一些电子密度较高的颗粒。结论:白果内酯可杀死卡氏肺孢子虫,经白果内酯治疗后肺部炎症减轻。Objective：To study the Pathological changes in lungs of rats infected with Pneumocystis carinii pneumonia after treatment with bilodalide （BL）. Methods：PCP rat models were established of hypodermic injection of dexamethasone twice a week for 6 weeks. The PCP rats were treated with BL 30mg/ （kg·d）× 8days and killed to obtain lungs. Pathological changes in lung tissues were observed under light and electron microscope .Infected and normal rats were established as controls. Results： The number of Pc cysts was markedly reduced in the lung print smear and inflammation in the lung was lightened. The investigation of thinner lung tissue section by transmission electronic microscope Showed that the Pneumocystis carinii treated with bilobalide had formation of vacuoles in trophozoites,swelling and destroying of cell organ, rupture of cytomembrane and some high density electronic granulae were investigated in the cytoplasm. Membrane of cyst was destroyed too. Conclusion：Bilodalide can kill Pc and alleviate inflammation in the lung tissues of PCP rats.

关 键 词：白果内酯 卡氏肺孢子虫肺炎 病理学 超微结构 

分 类 号：R363.1[医药卫生—病理学]