帕金森病大鼠黑质区神经干细胞分化的观察  

Experimental study on the differentiation of neural stem cells in substantia nigra of rat model of Parkinson's disease

作　　者：曾水林[1] 王磊[1] 朱建宝[1] 李涛[1] 刘卫国[2] 束汉生[3] 闫福麟[4] 

机构地区：[1]东南大学神经生物研究所,江苏南京210009 [2]南京市脑科医院 [3]安徽省蚌埠市第三人民医院脑外科 [4]东南大学附属中大医院神经内科

出　　处：《解剖与临床》2007年第1期27-30,共4页Anatomy and Clinics

基　　金：江苏省自然科学基金资助项目(BK2002056)

摘　　要：目的:探讨帕金森病(Parkinson’s disease,PD)大鼠中脑黑质区神经干细胞(NSCs)的分化情况。方法:将6-OHDA注入纹状体内制作PD大鼠模型。随机将成功模型分为3 d5、d7、d1、4 d2、8 d组,每组6只;另设假手术及正常对照组各3只。向成功模型鼠腹腔注射5-溴脱氧尿苷(Brdu)。用Brdu/NeuN、Br-du/GFAP、Brdu/TH免疫双标组织化学方法检测黑质区内源性NSCs向神经元、神经胶质细胞和多巴胺(DA)能神经元的分化情况。结果:PD模型7 d组,在黑质区Brdu+/GFAP+、Brdu+/NeuN+细胞开始出现,14 d组双标的阳性细胞数量逐渐增加,28 d达到高峰,14 d、28 d组与其它组比较差异显著(P<0.05)。在这些双标细胞中,Brdu+/GFAP+细胞数量较多,Brdu+/NeuN+细胞数量较少(P<0.05),未发现Brdu+/TH+细胞。结论:PD大鼠模型黑质区NSCs多数分化为神经胶质细胞,少数分化为神经元,在没有诱导和干预下,未见有向DA能神经元分化。Objective：To explore the differentiation of neural stem cells （NSCs）in substantia nigra of rat model of Parkinson＇ s disease（PD）. Methods： Rat model of PD was made by injecting the 6- hydroxydopominin（6- OHDA）in the striatum. The PD rats were divided into 3 d,5 d,7 d, 14 d and 28 d pups,each pup consisted of 6 rats. The sham operation group and normal control contained 3 rats respectively. The PD rats were killed at different time points, and their brain were taken out.The PD rats had been injected Brdu into abdomen for 14 days before they were killed.The ex- pression of Brdu/GFAP,Brdu/NeuN and Brdu/TH were detected by using immunocytochemistry method. The distinctive marker for Brdu/GFAP and Brdu/NeuN double labling method were used to detect the phenotype of the differentiated cells.Results：The number of Brdu ＋/GFAP ＋ and Brdu ＋/NeuN ＋ cells began to increase at 14th d,peaked at 28th d after PD model established. Compared with 3d,5d, 7d and control groups,the difference was significance（ P 〈 0.05）. There were more Brdu＋/GFAP^＋ cells than Brdu^＋/NeuN^＋ cells（ P 〈 0.05）,and not any Brdu ^＋/TH^＋ cells among them. Conclusion：The rat model of PD by direct injection of 6 - OHDA in the striatum may stimulate the proliferation of inherent NSCs,most of which differentiate to astrecytes and a few turned into neurons. No any dopaminergic neurons be observed if without induction and intervention.

关 键 词：帕金森病 神经干细胞 分化 黑质 免疫组织化学 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]