被动吸烟致金黄地鼠胎鼠眼畸形的形态学观察  

Morphological Study on Golden Hamster Eye Abnormalities Caused by Passive Smoking

作　　者：孟旭霞[1] 李玲[2] 谷方[3] 于向民[2] 

机构地区：[1]青岛大学医学院附属医院眼科,山东青岛266003 [2]青岛大学医学院组织胚胎学教研室,山东青岛266021 [3]青岛大学医学院解剖教研室,山东青岛266021

出　　处：《环境与健康杂志》2007年第2期90-92,F0003,共4页Journal of Environment and Health

基　　金：山东省自然科学基金资助项目(Y9813042)

摘　　要：目的观察被动吸烟致金黄地鼠胎鼠眼畸形的作用和畸形胚胎眼的形态学改变。方法将孕鼠随机分为吸烟组和对照组,吸烟组30只,分为3组,每组10只,于孕7、8、9d在本实验室自制的被动吸烟染毒箱中用香烟给予烟熏A对照组15只孕鼠也分为3组,除箱内无烟熏外,其他条件与吸烟组相同。于孕13d剖腹取胎观察眼畸形情况。结果对照组活胎141只,无眼畸形胎鼠。吸烟组活胎252只,眼畸形胎鼠26只,眼畸形发生率为10.3%。对照组与吸烟组的活胎数差异无统计学意义(t=1.46,P>0.05)。吸烟组总眼畸形发生率显著高于对照组(χ2=15.58,P<0.001)。组织学观察发现,部分胚胎无视泡、视杯结构;部分胚胎可见发育阶段的视泡、视杯残基;部分胚胎视杯形态异常。结论被动吸烟对胚胎鼠有眼致畸作用。香烟烟雾可阻碍或延迟视泡、视杯的发生和发育,这是被动吸烟致胚胎眼畸形的形态学基础。Objective To study the eye teratogenie effects of passive smoking on the golden hamster embryos and to observe the morphological changes of the abnormal eyes. Methods By using the self-made passive smoking cage ,the passive smoking models of pregnant gold hamsters were developed. On the 13th gestation day, the golden hamster fetuses were taken out to be continuously sliced and stained with HE to examine the development of eye. Results The eye malformation rate in the experimental group was 10.3% which was significantly higher than that in the control group （X^2=15.58,P〈0.001）. In the morphological observation, it was found that in experimental groups, parts of the fetuses had no optic vesicle and optic cup, parts had residual bases of these structures which stopped in different developing stages, and parts had optic cup with morphological abnormality. Conclusion Passive smoking has significant eye teratogenic effects on embryos. The smog of tobacco can retard or delay the formation and development of the optic vesicle and optic cup of the fetus, which is considered as the morphological base of the passive smoking-induced eye malformation.

关 键 词：烟草烟污染 眼 畸形 胚胎学 

分 类 号：R994.6[医药卫生—毒理学]