姜黄素对人膀胱癌T24细胞增殖的抑制作用  

The Study of Curcumin Influence on Proliferation of Human Bladder Cancer T24 Cells

作　　者：孔涛[1] 盛玉文[1] 姜恩魁[2] 

机构地区：[1]锦州医学院附属第一医院泌尿外科 [2]锦州医学院生理学教研室,辽宁锦州121000

出　　处：《锦州医学院学报》2006年第5期44-47,共4页Journal of Jinzhou Medical College

摘　　要：目的 本研究旨在探讨姜黄素对人膀胱癌T24细胞增殖的影响。方法 培养人膀胱癌T24细胞，施加不同浓度姜黄素（10，50，100，250，500μM），观察对细胞增殖的影响。应用四唑盐比色法（MTT法）观察细胞增殖抑制情况；应用彩色图像分析系统观察细胞形态学特征（HE染色）。结果 姜黄素各组较对照组增殖降低；姜黄素各组，存在剂量一效应关系（10～500μM）和时间-效应关系（12h，24h，48h，72h）。结论 姜黄素抑制人膀胱癌124细胞增殖。Objective The investigation studied curcumin influence on proliferation of human bladder cancer T24 ceils. Methods Human bladder cancer T24 ceil were educated. And then, different density （10, 50, 100, 250, 500μM） were added into above ceils and incubated to observe influence on proliferation. Ceil proliferation condition was observed by MTr. Ceil morphology character was compared by color image analysis system （ stained by hehematoxylin and eosin） . Results The condition of proliferation in cureumin groups decreased compared with the control group, there were dose - effect （ 10 - 500μM） and time - effect （ 12h, 24h, 48h, 72h） relation in diferent cureumin groups. Conclusions Curcumin can reduce human bladder cancer T24 ceils proliferation.

关 键 词：姜黄素 膀胱癌 T24细胞 增殖 

分 类 号：R737.14[医药卫生—肿瘤]