急性粒细胞白血病珠蛋白基因表达失衡的研究  

The Study of Disequilibrium of α/β GlobinGene Expression in Acute Myeloid Leukemia

作　　者：徐学明[1] 傅敏庄[1] 王葆春[1] 朱定尔[1] 

机构地区：[1]湖南医科大学分子生物学研究室

出　　处：《生物化学杂志》1996年第6期659-663,共5页

摘　　要：应用竞争性逆转录聚合酶链式反应（ＲＴ－ＰＣＲ）测定了１０例急性粒细胞白血病Ｍ＿２型（ＡＭＬ）患者外周血网织红细胞中α／β珠蛋白ｍＲＮＡ的相对含量，其中８例表现不同程度增高，２例正常，均值为１．５１３±０．１８２（±ｓ），与正常对照组（１．２４±０．０８３）进行ｔ检验比较，有非常显著差异（Ｐ＜０．０１）．此外，ＰＣＲ－ＳＳＣＰ分析显示ＡＭＬ患者β珠蛋白基因启动子区序列（５＇端－１３５至＋１２２位核苷酸）无明显异常。说明ＡＭＬ患者珠蛋白基因表达失衡系由于转录异常，很可能系ＡＭＬ的发生对α／β珠蛋白基因的平衡表达产生了某种影响，从而表现为获得性β地中海贫血特征。实验结果为进一步探讨白血病的发病机理及其α／β珠蛋白基因表达失衡的机制奠定了基础。The relative amounts of α/β globin mRNA ratio (α/βmRNA ratios) in peripheral reticulocytes from the patients with acute myeloid leukemia (AML) was quantitated by the reverse transcription-polymerase chain reaction(RT-PCR) techniques. The α/β mRNA ratios in ten patients with AML were determined to be 1. 513 ±0 . 182 ( mean ±SD), while those in normal group is 1.214±0 .083, the α/β mRNA ratios in AML patients differ from those in normal control with very significant difference (P<0.01) . The disequilibrium of α/β globin chain biosynthesis in different types of leukemia had been reported already by our laboratory before. The above results showed that the disequilibrium of globin biosynthesis in AML may be caused by the disturbance of globin gene expression. The characteristics of the expression of α/β globin genes in AML is similar to that in β-thalassemia and is called as'Acquired β-thalassemia' here, the mechanism of which is still unknown, and needs to be further studied in order to elucidate the pathogenesis of leukemia,as well as the regulation of the globin gene expression in leukemia.

关 键 词：白血病 粒细胞白血病 急性 珠蛋白 基因表达 

分 类 号：R733.710.2[医药卫生—肿瘤]