痤疮丙酸杆菌生物膜的构建  

The building of biofilm of Propionibacteria acnes

作　　者：齐显龙[1] 孙东杰[1] 徐修礼[1] 高剑[1] 林琨[1] 李春英[1] 高天文[1] 

机构地区：[1]第四军医大学西京医院,西安710032

出　　处：《中国麻风皮肤病杂志》2007年第2期126-128,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:构建痤疮丙酸杆菌的生物膜,明确其生长特性,以期更加深入了解痤疮丙酸杆菌的致病机制。方法:痤疮丙酸杆菌标准株及临床株分别接种厌氧肉汤,麦氏比浊法调整细菌浓度,接种96孔板,XTT法检测490nm吸光值,取平均值判定最佳成膜细菌浓度;菌液接种入装有盖玻片6孔板,厌氧培养,不同时间点进行扫描电镜(SEM)观察。结果:最佳成膜时间可以判定为接种后96h,最佳成膜浓度为1×108cfu/mL;两者形成生物膜的形态无明显差别。结论:本研究明确了痤疮丙酸杆菌的最佳成膜浓度,构建成其生物膜,为进一步研究痤疮丙酸杆菌奠定一定基础。Objective： To build the biofilm of Propionibacteria aches （ P. ache）, for further study in pathogenesis of P. aches. Methods： Clinical P. aches strains cultured from patients and standard strains were inoculated into anaerobe broth. Mac turbidimetric method was used to adjust bacteria density. After XTT staining, absorbency at 490 nm was read, the mean value was used to determine the best basteria density to form the biofilm. The bioflm of P. aches formed on coverslip with SEM was also observed. Results： The best time point for biofilm forming was 96 h after inoculation, while the best bacteria density for forming the biofilm was 1 × 10^8 cfu/mL. There was no difference in the shape of biofilm between the P. aches type strain （NCTC 737） and the strain from patients. Conclusion： This experiment has identified the best time and bacteria density to form the biofilm of P. acnes, and the biofllm of P. aches observed with SEM, which would be the base for further study on P. aches.

关 键 词：痤疮丙酸杆菌 生物膜 扫描电镜 吸光值 

分 类 号：R378[医药卫生—病原生物学]