树突状细胞在大鼠穿透性角膜移植排斥反应中的作用  

Role of dendritic cells in graft rejection after penetrating keratoplasty

作　　者：白浪[1] 陆晓和[1] 党森涛[1] 周瑾[1] 

机构地区：[1]南方医科大学珠江医院眼科,广东广州510282

出　　处：《南方医科大学学报》2007年第1期72-74,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(032839)~~

摘　　要：目的观察大鼠角膜组织中树突状细胞的分布,以了解树突状细胞在穿透性角膜移植排斥反应中的作用。方法制作大鼠穿透性角膜移植模型,观察角膜透明度、新生血管。采用免疫组织化学方法检测OX-62+树突状细胞在角膜的分布。结果术后角膜植片均出现不同程度的新生血管、角膜水肿、混浊、基质增厚。排斥反应发生率100%。正常角膜组织中未见OX-62+树突状细胞分布,在发生急性排斥反应的角膜上皮层中有阳性细胞表达。脾脏阳性细胞表达与文献报道一致。结论OX-62+树突状细胞出现在移植后发生排斥的角膜组织中,角膜组织中的树突状细胞可能参与角膜移植后的免疫反应。Objective To gain insight into the role of dendritic cells in graft rejection following penetrating keratoplasty by investigating their distribution in rat cornea. Methods Orthotopical corneal transplantation was performed and immunohistochemical staining of the whole-mount cornea and the spleen tissue specimen employed to determine the distribution of the dendritic cells in the cornea. Results Graft rejection occurred in all rats following the transplantation. No OX-62^＋ dendritic cells were found in normal cornea but they were present in the epithelium of the cornea graft with allograft rejection. Conclusion OX-62^＋ dendritic cells presenting in the rejected cornea may be related to acute graft rejection after penetrating keratoplasty.

关 键 词：树突状细胞 0X-62 角膜 移植排斥反应 免疫组织化学 

分 类 号：R779.65[医药卫生—眼科]