万寿菊根提取物对山楂叶螨谷胱甘肽S-转移酶和蛋白酶及蛋白质含量的影响  

Effects of Tagetes erecta extracts on glutathione S-transferase and protease activities and protein content in Tetranychus viennensis

作　　者：师光禄[1] 王有年[1] 王鸿雷[2] 赵莉蔺[2] 刘素琪[3] 曹挥[3] 于同泉[1] 路苹[1] 

机构地区：[1]北京农学院农业应用新技术北京市重点实验室,北京102206 [2]中国科学院动物研究所农业虫鼠害综合治理国家重点实验室,北京100080 [3]山西农业大学农学院,山西太谷030801

出　　处：《应用生态学报》2007年第2期400-404,共5页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30170759;30571506);北京市自然科学基金重点项目(6071001);北京市教委平台建设项目(2006);北京市科委区县专项资金项目(2006);北京市都市型果业学科与果树生态安全创新团队资助项目

摘　　要：采用常规生化实验方法,探讨了山楂叶螨在光、暗条件下经万寿菊根的氯仿提取物(TPC)作用后谷胱甘肽S-转移酶、蛋白酶活性及蛋白质含量.生物样品采用活体处理和离体处理相结合的方法.结果表明:万寿菊根氯仿提取物的光活化生物活性最高,其次为水提取物,最后为甲醇提取物;山楂叶螨经TPC处理后,谷胱甘肽S-转移酶和蛋白酶活性显著升高,蛋白质含量明显下降,蛋白酶及蛋白质含量变化程度在光照条件下显著高于黑暗处理.万寿菊根氯仿提取物中存在的活性物质,能够促进山楂叶螨离体酶液中蛋白酶的活化;TPC通过激活试螨体内的蛋白酶而促进蛋白质的降解.万寿菊次生物质的生物活性主要属于光活化活性.With in vivo and in vitro Tagetes erecta roots under light and dark as test materials, this paper studied the effects of their extracts on the glutathione S-transferase and protease activities and protein content in Tetranychus viennensis. The results showed that the chloroform extract of T. erecta roots had the highest light-activated activity, followed by water extract, and methanol extract. After treated with chloroform extract, the glutathione S-transferase and protease activities in T. viennensis increased markedly, while its protein content decreased obviously. The variation degree of T. viennensis protease activity and protein content was significantly higher when the chloroform extract came from the T. erecta roots under light, suggesting that there existed active matters in the extract, which could promote the activation of protease, and thus, the decomposition of protein in T. viennensis. The bioactivity of T. erecta metabolites was mainly of light-activated one.

关 键 词：万寿菊 山楂叶螨 谷胱甘肽S-转移酶 蛋白酶 蛋白质含量 

分 类 号：S482.3[农业科学—农药学]