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作 者:岑东芝[1] 李扬秋[1] 陈少华[1] 杨力建[1] 郁志[1]
机构地区:[1]暨南大学医学院血液病研究所,广州510632
出 处:《临床肿瘤学杂志》2007年第2期81-84,共4页Chinese Clinical Oncology
基 金:国家自然科学基金项目(39870358);广东省科技计划项目(2005B50301016);广州市科技计划项目(2003J1-10011;200521-E4015);血液病学国务院侨办重点学科基金资助项目
摘 要:目的:了解急性早幼粒细胞白血病(APL)患者外周血TCRVα亚家族T细胞的分布及其克隆性。方法:利用RT-PCR扩增9例APL患者和10例正常人外周血单个核细胞中29个TCRVα亚家族基因的互补决定区3(CDR3),了解TCRV理亚家族T细胞的分布;阳性产物进一步经基因扫描分析CDR3长度,从而了解其克隆性。结果:9例患者外周血T细胞所能检测到的Vα亚家族T细胞分别为1~9个亚家族,以Vet3和Vα19出现频率较高;克隆性增殖T细胞可在8例患者中检测到,以Vα3、Vα26和Vα27出现克隆性T细胞频率较高(3/8)。正常人外周血T细胞中表达绝大多数Vα亚家族,呈多克隆性增殖特点。结论:APL患者外周血Vα亚家族T细胞存在明显的倾斜性分布和克隆性增殖特点,后者可能是对APL细胞抗原的反应性增殖,是否具有特异性杀伤作用,有待进一步检测。Objective:To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complementary determining region 3 ( CDR3 ) of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by Genescan. Results: 1-9 subfamilies could be detected in peripheral blood T ceils from 9 cases with APL, the fre- quent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 (3 out of 8 cases). However, almost all Vα subfamilies with polyclonal expansion could be detected in Peripheral blood T cells from 10 cases of normal individuals. Conclusion:Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells are the common feature in patients with APL. Clonal expansion of T cells might reflect a re- sponse in host to APL cell associated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.
关 键 词:急性早幼粒细胞白血病 T细胞受体基因 克隆性
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