体外培养胃癌细胞的Ⅳ型胶原酶、ras-P_(21)及CEA表达研究  

STUDIES ON TYPE IV COLLAGENASE,RAS-P_(21) AND CEA EXPRESSION IN CULTURED HUMAN GASTRIC CANCER CELLS IN VITRO

作　　者：吴金莺[1] 李日乐[1] 吴信法[2] 

机构地区：[1]上海铁道大学附属甘泉医院中心实验室,上海200065 [2]上海铁道大学医学院基础医学部病理生理教研室,上海200070

出　　处：《上海铁道大学学报（医学版）》1996年第1期12-14,共3页

摘　　要：应用免疫细胞化学方法,研究人胃癌培养细胞中Ⅳ型胶原酶、ras—P_(21)及CEA分布与表达。结果显示,被测胃癌细胞中Ⅳ型胶原酶、ras—P_(21)、CEA均呈阳性,表明培养的胃癌细胞具有合成和异常表达Ⅳ型胶原酶、ras—P_(21)和CEA的作用。结合文献讨论了Ⅳ型胶原酶、ras—P_(21)、CEA与胃癌细胞浸润转移之间的相互关系。Immunocytochemistry and cell cultured techniques were used in this experiment to study the expression and distribution of type IV collagenase,ras-p21 and CEA in cultured human gastric cancer cells. The results showed that the positive expression of type IV collagenase. ras-p21 and CEA were observed in all detected gastric cancer cells. It is suggested that the cultured gastric cancer cells have the function of synthesis and abnormal expression type IV collagenase,ras-p21 and CEA. The relationship between type IV collagenase, ras-p21 and CEA and invasion and metastasis of gastric cancer cells were discussed in this artical.

关 键 词：胃肿瘤 癌胚抗原 细胞培养 CEA 

分 类 号：R735.2[医药卫生—肿瘤]