基质金属蛋白酶-9和表皮生长因子受体在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of MMP-9 and EGFR in patients with non-small cell lung cancer

作　　者：吴萍[1] 叶晓锋[1] 吕怀盛[1] 秦憬[1] 

机构地区：[1]宁夏医学院附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2007年第1期10-12,F0003,共4页Ningxia Medical Journal

摘　　要：目的探讨基质金属蛋白酶-9(MMP-9)和表皮生长因子受体(EGFR)在非小细胞肺癌(NSCLC)中的表达及与临床、病理的关系。方法对经随诊资料完整的术后非小细胞肺癌患者组织标本60例、癌旁组织20例和肺正常组织5例,采用S-P免疫组化法检测MMP-9和EGFR。结果60例NSCLC中MMP-9蛋白的阳性表达率为63.33%,20例癌旁组织的阳性表达率为10%,5例正常组织未见阳性染色细胞。EGFR的阳性表达率为65.00%,20例癌旁组织及5例正常组织未见阳性染色细胞。癌组织MMP-9和EGFR与癌旁组织相比差异有显著意义(P<0.05)。MMP-9的表达与肿瘤的TNM分期、淋巴结转移呈正相关(P<0.05),EGFR与肿瘤大小、TNM分期、病理分级、淋巴结转移有显著性差异(P<0.05)。MMP-9和EGFR的表达与患者的年龄、性别、吸烟习惯及肿瘤的病理类型间无显著性差异(P>0.05)。结论MMP-9和EGFR的信号传导对于非小细胞肺癌的侵袭和转移起着重要作用,MMP-9和EGFR的共同高表达是肺癌不良预后的指标。Objective To investigate the expression of MMP - 9 and EGFR in non - small cell lung cancer and they＇ re relationship between them and the prognostic significance.Methods The expression of MMP- 9 and EGFR were detected in 60 NSCLC samples, 20 lung tissues adjacent to cancer and 5 normal lung tissues as control by immunohistochemical technique （streptavidin - peroxidase conjugate method, S - P）.Resuits The positive expression of MMP - 9 and EGFR in 60 cases NSCLC was 63 . 33 % and65.00% respectively which was signiilcantly higher than that in 20 lung tissues adjacent to cancer and 5 normal lung tissues （ P 〈 0.05）. The MMP - 9 and EGFR positive expression were not correlated to age, sex, smoking histology and the pathologic types of tumor cell （ P 〉 0.05）, The positive expression of MMP - 9 protein was related with TNM staging and also related to lymph node metastasis （ P 〈 0.05）, The positive expression of EGFR protein was related with tumor magnitude, cell differentiation and also related to TNM staging , lymph node metastasis （ P 〈 0.05）. Conclusion MMP - 9 and EGFR may play important roles in NSCLE invasion and metastasis. Over expression of MMP - 9 and EGFR could act as a reference to evaluate metastasis and unfavorable prognosis of NSCLC.

关 键 词：基质金属蛋白酶-9 表皮生长因子受体 非小细胞肺癌 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]