应用微透析技术活体检测帕金森病动物模型的神经生化改变  

^In vivo neurochemical changes in Parkiuson's animal models measured by microdialysis

作　　者：刘振国[1,2] 陈生弟[1,2] 余慧贞[1,2] 唐琴梅[1,2] 张可佳[1,2] 徐修容[1,2] 

机构地区：[1]上海第二医科大学附属瑞金医院神经内科 [2]中国科学院上海药物研究所

出　　处：《中华老年医学杂志》1996年第6期364-366,共3页Chinese Journal of Geriatrics

摘　　要：目的以活体检测探讨帕金森病（ＰＤ）猴模型神经递质含量的改变。方法应用１－甲基－４－苯基－１，２，３，６－四氢吡啶（ＭＰＴＰ）制备偏侧ＰＤ猴模型，采用颅内微透析技术结合高效液相色谱－电化学方法（ＨＰＬＣ－ＥＣ）活体检测双侧尾核区多巴胺（ＤＡ）及其代谢产物的含量。结果ＭＰＴＰ注射侧与注射对侧相比，尾核区ＤＡ、３，４－二羟基苯乙酸（ＤＯＰＡＣ）和高香草酸（ＨＶＡ）的含量分别减少７０％、３４％和３８％。结论颅内微透析技术是活体检测脑内神经递质含量的最有效方法。Objective To measure the extracelluar transmitters in monkey model of Parkinsonism in vivo.Methods Unilateral intracarotid infusion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) produces a useful monkey model of hemiparkinsonism. Brain microdialysis,combined with high performance liquid chromatography and electrochemcal detection (HPLC-ECD), was employed to measure the levels of dopamine(DA)and its metabolites in the bilateral caudate nuclei of hemiparkinsonian monkey. Results The levels of DA,DOPAC and HVA in MPTP-treated side were decreased by 70%, 34% and 38% respectively,as compared with those in the untreated side. Conclusions The result suggests that brain microdialysis is a valuable tool for measuring in vivo neurochernical changes,and it helps to comprehend the relationship between symptoms of Parkinsonism and changes of the extracelluar neurotransmitters.

关 键 词：检测 微透析 帕金森病 神经生化改变 

分 类 号：R742.504[医药卫生—神经病学与精神病学]