靶向survivin基因的siRNA对大肠癌细胞生物学特性的影响  

Influence of survivin-targeted siRNA on the biological features of colorectal carcinoma cells

作　　者：熊英[1] 郭文[1] 李霆[1] 

机构地区：[1]南方医科大学南方医院消化病研究所,广州510515

出　　处：《广东医学》2007年第3期342-344,共3页Guangdong Medical Journal

基　　金：广东省社会发展领域科技计划项目(编号:63005)

摘　　要：目的向大肠癌细胞Lovo中导入靶向survivin基因的siRNA,研究其对Lovo细胞生物学效应影响。方法设计2条特异性靶向survivin基因的siRNA,体外转录法合成,通过脂质体导入Lovo细胞并检测转染后细胞内survivin基因表达水平及凋亡、增殖情况。结果细胞内survivin mRNA表达水平降低,细胞凋亡率升高,增殖能力减弱。结论所设计的靶向survivin基因的siRNA能有效降低目的基因的mRNA表达,诱导细胞凋亡,抑制细胞生长。为大肠癌基因治疗的可行性提供了有力的证据。Objective To study the transfection of survivin - targeted siRNA to Lovo cells and its influence on biological features. Methods Two pairs of21bp siRNAs specific target survivin gene were designed and synthesized by in vitro transcription. After transient transfection of the two survivin - targeted siRNA into Lovo cells by Lipofectamine 2000, the expression of survivin level, apoptosis and cell proliferation were investigated. Results The expression level of survivin mRNA in the ceils was significanty reduced. The ratio of apoptosis significantly in creased and the abitily of proliferation was significantly reduced. Conclusion The two survivin - targeted siRNAs can successfully suppress the expression of survivin mRNA, inhibit cell growth and induce cell apoptosis. It provides a powerful evidence for colorectal carcinoma gene therapy.

关 键 词：SURVIVIN 基因治疗 RNA干扰 细胞生物学特性 

分 类 号：R394[医药卫生—医学遗传学]