L-半胱氨酸产生菌TS1138的补料分批发酵动力学研究  

STUDY ON FED-BATCH FERMENTATION KINETICS OF L-CYSTEINE PRODUCING PSEUDOMONAS STRAIN TS1138

作　　者：怀丽华[1] 周昌平[1] 陈宁[1] 杨文博[2] 白钢[2] 

机构地区：[1]天津科技大学生物工程学院 [2]南开大学生命科学学院,天津300071

出　　处：《河南工业大学学报（自然科学版）》2007年第1期32-35,共4页Journal of Henan University of Technology：Natural Science Edition

基　　金：天津市科委应用基础研究重点项目(05YFJZJC00901)

摘　　要：以DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-2-amino-Δ-2-thiazoline-4-carboxylic acid,DL-ATC)为底物,采用来自假单胞菌(Pseudomonas sp.)TS1138的L-半胱氨酸合成酶系对DL-ATC进行生物转化合成L-半胱氨酸.以5 L发酵罐补料分批发酵试验数据为依据,对TS1138菌株的补料分批发酵动力学进行了研究,建立了菌体生长、产物形成和基质消耗的动力学模型,并应用MATALAB软件进行模型拟合,拟合模型较好地反映了L-半胱氨酸产生菌TS1138的补料分批发酵过程.L-cysteine was produced by means of microbial transformation of DL-2-amino-Δ^2-thiazoline-4-carboxylic acid （DL-ATC） in the presence of L-cysteine synthesizing enzyme from Pseudomonas sp. TS1138. Based on the experimental data of fed-batch fermentation in 5 L fermentation reactor, the kinetic model of cell growth, enzyme production and substrate consumption were constructed. Three kinetic models were in good agreement with the experimental results through proper analysis by software MATALAB. The kinetic models appeared to provide a reasonable description for each parameter in the fermentation.

关 键 词：L-半胱氨酸 动力学模型 MATLAB 假单胞菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]