Nogo-A mRNA在缺氧缺血性脑损伤新生大鼠脑组织中的表达  

Expression of nerve growth inhibitor Nogo-A mRNA in the brain of newborn rats with hypoxic-ischemic brain damage

作　　者：刘仁红[1] 周晓光[1] 熊爱华[2] 

机构地区：[1]广州医学院第二附属医院新生儿科,510260 [2]暨南大学药学院

出　　处：《中华围产医学杂志》2007年第1期37-40,73,共5页Chinese Journal of Perinatal Medicine

基　　金：广东省医学科学技术研究基金资助项目（A2004305）

摘　　要：目的研究神经生长抑制因子Nogo-A mRNA在缺氧缺血性脑损伤（hypoxic-ischemic brain damage，HIBD）新生大鼠脑组织的动态变化，探讨Nogo-A基因对HIBD新生大鼠中枢神经再生的影响。方法将180只SD新生大鼠随机分为对照组（n=90）和实验组（n=90），实验组制成HIBD模型，根据处死动物的时间不同随机分为HIBD后3、7、14、21和28d共5个亚组，每组18只；对照组也在相应时间点取材；采用原位杂交法检测新生大鼠脑组织Nogo-A mRNA在不同时间的表达量。结果原位杂交法显示Nogo-A mRNA在实验组新生大鼠的皮层呈强阳性表达，阳性细胞分布密集，对照组阳性细胞分布较实验组稀疏；实验组新生大鼠脑组织Nogo-A mRNA阳性细胞数量在缺血缺氧后7d（34．73±3．34）比14d（36．67±5．33）和21d（42．33±2．21）均较对照组7d（28．67±2．91）、14d（32．17±5．06）和21d（36．83±4．90）明显增高（P〈0．05或0．01）。结论脑组织Nogo-A mRNA表达在脑损伤急性期（3d）变化不明显，而在1周后较为明显，提示缺氧缺血性脑损伤后期Nogo-A mRNA表达明显升高，它可能是造成新生大鼠脑损伤后神经再生障碍的重要原因之一。Objective To study the changes of nerve growth inhibitor Nogo-A mRNA in the brain of newborn rats with hypoxic-ischemic brain damage （HIBD） and its effects on regeneration of central nervous system. Methods One hundred and eighty newborn rats were divided into experiment group （n=90） and control group （n=90）. Newborn rats in experiment group were established HIBD model Situ-hybridization was used to detect the expression of Nogo-A mRNA in the brain of all rats at different time after HIBD or birth. Results Situ-hybridization showed that, Nogo-A positive cells in cerebral cortex in experiment group were stronger and densely covered than in control group. In experiment group, the number of Nogo-A positive cells at cerebral cortex at 7 days after hypoxic and ischemic （34.73±3.34）, 14 days （36.67±5.33）, and 21 days （42.33±2.21） were more than that at 7 days （28.67±2.91）, 14 days （32.17±5.06） and 21 days （36.83±4.90） in control group. There was no statistical difference between 21^th day and 28^th day （P〉0.05）. Comparing with control group, the number of Nogo-A positive cells in experiment group at 3, 7, 14 and 21 days were higher （P〈0. 05）. Conclusions The expression of Nogo-A mRNA in cerebral cortex changed slightly at the first period（3d） after hypoxic and ischemic but obviously after one week. Which indicated that dramatically increased Nogo-A mRNA in the latter period after hypoxic and ischemic may be one of the important causes of the central nervous system regeneration obstacle in the HIBD newborn rats.

关 键 词：缺氧缺血脑损伤 髓磷脂蛋白质类 基因表达 新生大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]