标记TRAILR地高辛探针及原位杂交检测喉癌组织mRNA  

The Determination of TRAILR mRNA in Human Squamous Carcinoma of Larynx with DIG-labeled TRAILR Probe and in situ Hybridization

作　　者：王晓云[1] 李家栋[1] 陈乾美[1] 耿勇[2] 赵厚育[1] 

机构地区：[1]贵阳医学院附院耳鼻咽喉科,贵州贵阳550004 [2]中国人民解放军第四十四医院耳鼻咽喉科,贵州贵阳550006

出　　处：《贵阳医学院学报》2007年第1期19-23,28,共6页Journal of Guiyang Medical College

基　　金：贵州省省长基金资助项目(S2000-3)

摘　　要：目的:标记肿瘤坏死因子相关凋亡诱导配体受体(TRAILR)地高辛(DIG)探针,检测喉癌组织中TRAILR mRNA,并探讨其意义。方法:采用RT-PCR原位杂交法检测喉癌组织TRAILR mRNA。结果:TRAILR地高辛探针标记成功;喉癌和正常对照中死亡受体4、5(DR4,DR5)mRNA呈强阳性,阳性率均为100%(34/34,5/5);正常对照中诱捕受体2(DcR2)mRNA呈强阳性,阳性率100%(5/5);喉癌中DcR2 mRNA可检出,阳性率14.71%(5/34);DcR2 mRNA主要分布在癌巢组织边缘,强度由癌巢中央到癌巢边缘逐渐加强。结论:TRAIL受体基因可能在喉鳞状细胞癌凋亡调控机制中发挥重要作用。Objective： To detect the Tumor necrosis factor-related apoptosis-inducing ligand （TRAILR） mRNA in human squamous carcinoma of larynx tissue and in normal larynx tissue with DIG-labelled TRAILR probe. Methods： TRAILR mRNA were tested by means of RT-PCR and in situ hybridization （ISH）. Results： （ 1 ） Both the DR4 and DR5 mRNA were strongly positively detected in all tumor samples and normal samples with same expression level ; （2） Death decoy receptors （DcR2） mRNA were positively detected in all normal samples, but in only 5 of the tumor samples （ 14.71%, 5/34）, and were mainly detected at the edge of the cancer nest. （3） In tumor samples, the expression level of DcR2 mRNA was different from the expression levels of DR4 and DtL5 （P 〈0. 01 ）. Conclusions： An ISH methods for TRAILR mRNA detecting in human squamous carcinoma of larynx has been successfully important role in established. The findings suggest that expression of different receptors might play an the apoptosis regulation of human squamous carcinoma of larynx.

关 键 词：喉肿瘤 地高辛 DNA探针 原位杂交 MRNA 肿瘤坏死因子相关凋亡诱导配体受体 鳞状细胞癌 

分 类 号：R739.65[医药卫生—肿瘤]