人成釉细胞瘤裸鼠皮下移植瘤模型的建立  

Establishment of Subcutaneous Xenotransplanted Tumor Model of Human Ameloblastoma in Nude Mice

作　　者：张磊涛[1] 黄洪章[1] 曾东林[1] 张彬[1] 

机构地区：[1]中山大学附属第二医院口腔颌面外科,广东广州510120

出　　处：《华西口腔医学杂志》2007年第1期12-14,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30471896)

摘　　要：目的建立人成釉细胞瘤的裸鼠皮下移植瘤模型。方法采用组织块直接贴壁法将新鲜成釉细胞瘤组织进行原代细胞培养,反复贴壁法和胰酶消化法使之纯化。然后将纯化的成釉细胞瘤细胞接种于裸鼠颈背部皮下。观察接种成瘤后瘤组织大体生长情况以及常规病理切片观察瘤细胞的侵袭生长情况。结果成釉细胞瘤细胞可以在裸鼠皮下存活,接种后第23天见有移植瘤形成,成瘤率为25%。病理切片显示瘤细胞可向裸鼠肌间生长,保持了其侵袭性生长的特性。结论初步建立了人成釉细胞瘤的裸鼠皮下移植瘤模型,为进一步研究成釉细胞瘤的侵袭生长特性奠定了基础。Objective To establish an subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice. Methods Ameloblastoma cells were absorbed by primary culture, repeat attachment and pancreas proteolytic enzyme were both used to purify them. Then, the purified cells were implanted subcutaneously into the nude mice. The specimens were respectively investigated by microscope in different spots after implanting. Results Ameloblastoma ceils can survive in all of the 8 nude mice. The xenograft can be found on 23 days after implanting. The rate of successful inocutation is 25%. The subcutaneously xenotransplanted tumor cells can be found with microscope in the inter-muscle tissues of nude mice. Conclusion The subcutaneously xenotransplanted tumor model of human ameloblastoma in nude mice was successfully established and it may benefit to further studies on this tumor.

关 键 词：成釉细胞瘤 裸鼠 移植瘤模型 

分 类 号：R739.8[医药卫生—肿瘤]