免疫抑制剂对IL-2致痫大鼠脑内谷氨酸及IL-2受体表达的影响  

Effects of Immunosuppressants on the Expression of Glu and IL-2R in Brain of Rats with Seizure Induced by IL-2

作　　者：卫向红[1] 李正莉[1] 向世强[2] 

机构地区：[1]华中科技大学同济医学院基础医学院解剖学系,武汉430030 [2]武汉科技大学医学院人体解剖学教研室,武汉430080

出　　处：《华中科技大学学报（医学版）》2007年第1期10-12,22,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2004ABA196);武汉科技大学校基金资助项目(No.2004XY36)

摘　　要：目的研究免疫抑制剂对白细胞介素-2(IL-2)诱导大鼠急性癫痫的抑制作用,及其对大脑皮质及海马内谷氨酸(Glu)I、L-2受体(IL-2R)表达的影响,探讨免疫抑制剂抗痫效应及其作用机制。方法将大鼠随机分为4组:A组(生理盐水对照组)、B组(IL-2组)、C组(环孢素-A+IL-2组)、D组(糖皮质激素处理组),对大鼠行侧脑室注射相应试剂后观察并记录大鼠行为表现,采用免疫组织化学方法检测大脑皮质及海马内Glu的含量及IL-2R的表达。结果A组无明显痫样发作;B组痫样发作程度达Ⅲ级;C组发作程度为Ⅰ级;D组无痫样发作。免疫组化染色显示,各组注药后2h,B组的大脑皮质及海马内GluI、L-2R免疫反应较A组明显增强,差异具有显著性意义。C组与A组相比,GluI、L-2R免疫反应无明显变化。D组与A组相比,GluI、L-2R免疫反应减弱。结论IL-2具有致痫效应,免疫抑制剂具有一定的抗痫作用,其机制与抑制Glu和IL-2R表达有密切关系。Objective To explore the anti-epileptic effect of immunosuppressants of glutamate （Glu） and interleukin-2 receptor （IL-2R） of cerebral cortex and hippocampal. Methods The rats were randomly divided into 4 groups： group A, normal saline control group; group B, IL-2 group; group C, cyclosporine （CsA）＋IL-2 group; group D, glucocorticoid （GC） group. The behavior was observed after injecting IL-2 into the lateral ventricle of rats and the expression of Glu and IL-2R of cerebral cortex and hippocampal was detected by using immunohistochemical staining. Results There was no epilepsy in groups A and D. The rats in group B showed epileptic activities about gradeⅢ. The seizure was partly restrained in rats of group C, showing grade Ⅰ epileptic activities. The immunohistochemical staining revealed that, 2 h after injection, the immunoreactive （IR） positive cells of Glu and IL-2R in cerebral cortex and hippocampal of group B were significantly increased as compared with group A. There was no significant difference in IR positive cells of Glu and IL-2R between group C and group A. Conclusion IL-2 could induce the epileptic seizure. The immunosuppressants could restrain the seizure, and its mechanism might be related to the inhibition of the expression of Glu and IL-2R.

关 键 词：癫痫 免疫抑制剂 谷氨酸 白细胞介素-2受体 

分 类 号：R742.1[医药卫生—神经病学与精神病学]