杜仲叶中绿原酸的提取纯化研究  

Study on the Extraction and Purification of Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves

作　　者：袁华[1] 邓良[1] 杨小俊[1] 闫志国[1] 吴元欣[1] 

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430073

出　　处：《时珍国医国药》2007年第2期446-448,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的研究杜仲叶中绿原酸的提取纯化方法。方法采用水提、絮凝脱色、树脂吸附、重结晶工艺提取纯化。结果杜仲叶的水浸提液浓缩后用1%壳聚糖絮凝去杂、活性碳脱色得到进样液,进样液用NKA-9树脂吸附,50%乙醇解吸,洗脱液浓缩后的粗品经甲醇重结晶得到纯度≥97%的绿原酸,提取率≥65%。结论优化完善了杜仲叶中绿原酸的提取纯化方法,为杜仲资源的充分综合利用和产业化开发提供了参考。Objective To study the optimum extraction and purification process for chlorogenic acid in Eucommia ulmoides Oliv. Leaves. Methods The process was carried out by water extraction, 1% chitosan flocculation, actived char decolorization, NKA- 9 resin adsorption, 50% ethanol desorption and methanol recrystallization. Results The extracting ratio of chlorogenic acid was 68% with purity of 97%. Conclusion An optimum and improved technology for the extraction and purification of chlorogenic acid in Ettcommia ulmoides Oliv. Leaves was investigated. It is helpful to industrial production of chlorogenic acid.

关 键 词：绿原酸 提取 纯化 杜仲叶 

分 类 号：R284.2[医药卫生—中药学]