苯丁酸钠联合吉非替尼对NB4细胞生物学活性的影响  

The effect of sodium phenylbutyrate combined with gefitinib on the biologic activity of tumor cells

作　　者：王旭[1] 李薇[1] 王冠军[1] 

机构地区：[1]吉林大学第一医院血液肿瘤科,吉林长春130021

出　　处：《中国老年学杂志》2007年第4期315-317,共3页Chinese Journal of Gerontology

基　　金：吉林省计委资助项目(20031268)

摘　　要：目的探讨联合应用苯丁酸钠(SPB)与吉非替尼对NB4细胞的抑制效果。方法将NB4细胞随机分成4组,即对照组,SPB组、吉非替尼组、和SPB组+吉非替尼组,细胞生长7d后应用MTT法检测药物对细胞的抑制率,并应用流式细胞仪检测细胞的凋亡情况,应用Westernblot的方法检测p21WAF1、CDK4、野生型p53蛋白的表达,并记录细胞的生长曲线。结果联合用药组肿瘤细胞受到明显抑制,凋亡率增加,p53及p21WAF1表达增加,CDK表达下降。与对照组,SPB组、吉非替尼组相比,差异均有统计学意义(P<0.05)。结论联合用药效果要比单纯用药效果明显,可以使肿瘤细胞受到明显抑制,凋亡率增加,其机制是通过p53依赖的p21WAF1的激活进而抑制周期素-周期素依赖激酶(cyclinD1-CDK4)复合物,从而阻滞了肿瘤细胞的增殖周期进行的。Objective To investigate the inhibitive effect of sodium phenylbutyrate （SPB） combined with gefitinib on NB4 cells. Methods NB4 ceils were randomly divided into control group, SPB group, gefitinib group and combination group. The inhibitive rate was detected by MTT 7 d after cellular growth, the apoptosis of NB4 was detected by flow cytometry, the expression of P21WAF1, CDK4 and p53 were detected by western blot and the growth curve was recorded. Results The NB4 ceils were suppressed obviously, the rate of apoptosis increased, the expression of p53 and P21WAF1 raised and the expression of CDK4 descended in combination group comparing to those in control, SPB and gefitinib group（ P 〈 0.05）. Conclusions The curative effect of sodium phenylbutyrate combined with gefitinib is more evident than that of SPB or gefitinib only, the mechanism of which is suppressing cyclinD1-CDK4 complex through activating P21WAFI depended by p53 to blnek proliferation cycle of tumor ceils.

关 键 词：苯丁酸钠 吉非替尼 联合用药 NB4细胞 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]