维生素E对培养心肌细胞脂质过氧化损伤的保护  

THE PROTECTIVE EFFECT OF VITAMIN E ON LIPID PEROXIDATION IN CULTURED HEART CELLS OF NEONATAL RATS

作　　者：王光武[1] 赵轶卓[1] 王凡[1] 赵志涛[1] 

机构地区：[1]白求恩医科大学地方病研究所,中国医学科学院心血管病研究所

出　　处：《营养学报》1996年第3期284-287,共4页Acta Nutrimenta Sinica

基　　金：国家"八五"攻关课题

摘　　要：应用氢过氧化枯烯引发体外培养的乳鼠心肌细胞脂质过氧化损伤过程，观察了添加维生素Ｅ（２００ｍｇ／Ｌ的α－生育酚）的影响。结果显示：维生素Ｅ能明显减轻细胞脂质过氧化物的蓄积、细胞内肌酸激酶漏出和ＤＮＡ断裂，保护细胞膜流动性、肌球蛋白轻链肌酸激酶和头部ＡＴＰ酶的活性；但对￣３Ｈ－ＴｄＲ掺入的影响不显著。表明维生素Ｅ具有抵抗脂质过氧化所致心肌细胞损伤的作用。ipid peroxidation in heart cells of neonatal rats was induced by cum-ene hydroperoxide,and the protective effect of vitamin E(VE)was invest-igated.The results showed that VE (200mg/L α-tocopherol)could abate the increase of lipid peroxide P<0.05 or 0.01),the leakage of intracellular cr-eatine phobphokinase(P<0.05) and the breakage of DNA,Study also proved that VE could protect the cell membrane fluidity,relieve the decrease of activities of creatine phosphokinase in myosine light chain (P<0.01)and adenosine triphosphatase in myosine head(P<0.01);but had a slight effect on ￣3H-TdR incorporation into DNA(P<0.05).It is suggested that VE is effective on protection of tnyocytes from injury caused by lipid peroxidation.

关 键 词：维生素E 脂质过氧化 心肌细胞 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R542.205[医药卫生—公共卫生与预防医学]