9-顺式维甲酸对胃癌细胞周期及周期因子表达的影响  

Effects of 9-cis retinoic acid on cell cycle and expression of cell cycle regulation factors in human gastric cancer cell line MGC80-3

作　　者：周艳红[1,2] 徐瑞成[1] 呼文亮[1] 

机构地区：[1]武警医学院细胞生物学教研室 [2]唐山职业技术学院医学院基础部,唐山063000

出　　处：《肿瘤》2007年第2期92-95,共4页Tumor

基　　金：天津市自然科学基金项目(编号:013804311)

摘　　要：目的探讨9-顺式维甲酸(9-cisRA)抑制胃癌细胞生长及其机制。方法RT-PCR检测9-cisRA作用MGC80-3细胞前后细胞周期蛋白D1(CyclinD1)和周期蛋白依赖性激酶4(CDK4) mRNA表达的变化;流式细胞术和MTT法检测其对细胞周期和生长抑制的作用;电镜观察细胞形态学改变。结果10μmol/L9-cisRA作用MGC80-3细胞96h时,CyclinD1和CDK4 mRNA表达均显著下降(P<0.01);9-cisRA对MGC80-3细胞生长抑制作用呈时间和剂量依赖性;9-cisRA诱导MGC80-3细胞阻滞于细胞周期的G1期,并呈典型的凋亡特征性的“亚G1峰”和形态学改变。结论9-cisRA对MGC80-3细胞有显著的生长抑制和诱导凋亡作用,与抑制CyclinD1和CDK4表达将细胞周期阻滞于G1期有关。Objective： To investigate the anti-tumor effects of 9-cis retinoic acid （9-cisRA） against gastric cancer and the corresponding mechanism. Methods： The mRNA expressions of cyclin DI and cyclin-dependent kinase 4 （CDK4） were detected by reversed transcriptase-polymerase chain reaction （RT-PCR） before and after MGC80-3 cells were treated with 9-cisRA. Effects of 9-cisRA on cell cycle and proliferation of MGC80-3 cells were examined by flow cytometry （FCM） and MTT assay, respectively. Morphological changes of KM3 cells were observed under transmission electric microscope. Results： Treatment with 9-cisRA 10 μmol/L for 96 h significantly down-regulated Cyclin DI and CDK4 mRNA expressions in MGC80-3 cells （ P 〈0.01 ）, inhibites the proliferation of MGCS0-3 cells in a dose- and time-dependent manner, and induces G1 phase arrest of MGC80-3 ceils. Typical apoptotic features were observed such as chromatin condensation and sub G1 peak. Conclusion： 9-cisRA inhibited the growth of MGC80-3 cells and induces apoptosis. It were related with down-regulation of CyclinD1 and CDK4 and arresting MGC80-3 cells at G1 phase.

关 键 词：胃肿瘤 9-顺式维甲酸 细胞周期 细胞周期蛋白DI MGC80-3细胞 

分 类 号：R735.1[医药卫生—肿瘤]