氟达拉滨对多发性骨髓瘤细胞系KM3细胞分泌IL-6的影响  

Effect of fludarabine on interleukin-6 (IL-6) secreted by multiple myeloma KM3 cells

作　　者：彭志刚[1] 周亚丽[1] 黄潇[1] 罗军[1] 赖永榕[1] 

机构地区：[1]广西医科大学第一附属医院血液内科,南宁530021

出　　处：《肿瘤》2007年第2期147-149,共3页Tumor

摘　　要：目的研究氟达拉滨(fludarabine)在体外对多发性骨髓瘤细胞KM3细胞生长的影响及对细胞上清液IL-6、sIL-6R的影响。方法采用MTT法及细胞计数法分别观察不同浓度氟达拉滨对KM3细胞生长的影响;用双抗夹心法检测不同浓度氟达拉滨作用KM3细胞后上清液白细胞介素6(interleukin-6,IL-6)和可溶性白细胞介素6受体(soluble interleukin-6 receptor,sIL-6R)的表达水平。结果MTT法及细胞计数法均显示氟达拉滨对KM3细胞有明显的抑制作用,氟达拉滨作用KM3细胞72h后,25、50、100、200、400nmol/L组的增殖抑制率均高于对照组(P<0.01),且与浓度成正比。400nmol/L组对KM3细胞增殖抑制率随时间的延长而增加。双抗夹心法检测结果显示KM3细胞经氟达拉滨作用96h后,其IL-6水平先升高后明显下降,而sIL-6R水平随药物浓度的升高而逐渐下降。结论氟达拉滨能明显抑制KM3细胞的增殖,同时能抑制KM3细胞自分泌IL-6和sIL-6R。。Objective：To investigate the effect of fludarabine on interleukin-6 （IL-6） secreted by multiple myeloma KM3 cells. Methods：The effects of fludarabine on the proliferation of multiple myeloma KM3 cells were examined by MTT assay and flow cytometry. The levels of IL-6 and soluble interleukin-6 receptor （sIL-6R） in the supernatant after incubation with different concentrations of fludarabine were tested by enzyme-linked immunosorbant assay （ELISA）. Results： Both MTT assay and flow cytometry showed that fludarabine had significant inhibitory effects on the growth of KM3 cells in vitro. Treatment with fludarabine 25, 50, 100,200,400 nmol/L for 72 h inhibited KM3 cells proliferation in concentration-dependent manner compared with control （ P 〈 0.01 ）. Fludarabine 400 nmol/L inhibited KM3 cells proliferation in a time-dependent manner. ELISA revealed that the level of IL-6 increased first and then significantly decreased after KM3 cells were incubated with fludarabine for 96 h and the level of sIL-6R decreased gradually coupled with the increase in the concentration of fludarabine. Conclusion： Fludarabine significantly inhibites the proliferation of KM3 cells and simultaneously decreases the autocrine of IL-6 and sIL-6R by KM3 cells.

关 键 词：多发性骨髓瘤 白细胞介素6 氯达拉滨 KM3细胞 

分 类 号：R733[医药卫生—肿瘤]